Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes

Natalia A. Moroz; Stefanie M. Novak; Ricardo Azevedo; Mert Colpan; Vladimir N. Uversky; Carol C. Gregorio; Alla S. Kostyukova

doi:10.1074/jbc.M112.434522

Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes

Natalia A. Moroz
, Stefanie M. Novak
, Ricardo Azevedo
, Mert Colpan
, Vladimir N. Uversky
, Carol C. Gregorio
, Alla S. Kostyukova

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: Tropomodulin is a tropomyosin-dependent actin-capping protein. Results: Mutations intropomodulin-1 that reduce its affinity for tropomyosin (R11K, D12N, Q144K) reduced inhibition of actin pointed-end polymerization in vitro and decreased assembly of tropomodulin-1 in skeletal myocytes. Conclusion: The tropomyosin-binding ability of tropomodulin-1 directly influences its actin filament regulatory activity. Significance: Creating a tool for studying the roles of different tropomodulin isoforms in living cells.

Original language	English
Pages (from-to)	4899-4907
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M112.434522
State	Published - 15 Feb 2013
Externally published	Yes

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title = "Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes",

abstract = "Background: Tropomodulin is a tropomyosin-dependent actin-capping protein. Results: Mutations intropomodulin-1 that reduce its affinity for tropomyosin (R11K, D12N, Q144K) reduced inhibition of actin pointed-end polymerization in vitro and decreased assembly of tropomodulin-1 in skeletal myocytes. Conclusion: The tropomyosin-binding ability of tropomodulin-1 directly influences its actin filament regulatory activity. Significance: Creating a tool for studying the roles of different tropomodulin isoforms in living cells.",

author = "Moroz, \{Natalia A.\} and Novak, \{Stefanie M.\} and Ricardo Azevedo and Mert Colpan and Uversky, \{Vladimir N.\} and Gregorio, \{Carol C.\} and Kostyukova, \{Alla S.\}",

year = "2013",

month = feb,

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doi = "10.1074/jbc.M112.434522",

language = "English",

volume = "288",

pages = "4899--4907",

journal = "Journal of Biological Chemistry",

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T1 - Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes

AU - Moroz, Natalia A.

AU - Novak, Stefanie M.

AU - Azevedo, Ricardo

AU - Colpan, Mert

AU - Uversky, Vladimir N.

AU - Gregorio, Carol C.

AU - Kostyukova, Alla S.

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Background: Tropomodulin is a tropomyosin-dependent actin-capping protein. Results: Mutations intropomodulin-1 that reduce its affinity for tropomyosin (R11K, D12N, Q144K) reduced inhibition of actin pointed-end polymerization in vitro and decreased assembly of tropomodulin-1 in skeletal myocytes. Conclusion: The tropomyosin-binding ability of tropomodulin-1 directly influences its actin filament regulatory activity. Significance: Creating a tool for studying the roles of different tropomodulin isoforms in living cells.

AB - Background: Tropomodulin is a tropomyosin-dependent actin-capping protein. Results: Mutations intropomodulin-1 that reduce its affinity for tropomyosin (R11K, D12N, Q144K) reduced inhibition of actin pointed-end polymerization in vitro and decreased assembly of tropomodulin-1 in skeletal myocytes. Conclusion: The tropomyosin-binding ability of tropomodulin-1 directly influences its actin filament regulatory activity. Significance: Creating a tool for studying the roles of different tropomodulin isoforms in living cells.

UR - https://www.scopus.com/pages/publications/84874061867

U2 - 10.1074/jbc.M112.434522

DO - 10.1074/jbc.M112.434522

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AN - SCOPUS:84874061867

SN - 0021-9258

VL - 288

SP - 4899

EP - 4907

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 7

ER -

Alteration of tropomyosin-binding properties of tropomodulin-1 affects its capping ability and localization in skeletal myocytes

Abstract

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