Abstract
Enzymatic methylation of histamine and analogues by histamine methyltransferase (HMT) was studied. Incubation of histamine or alpha-methylhistamine with HMT and S-adenosylmethionine was shown by GC-MS to yield tele-methyl and alpha-methyl-tele-methyl-histamine, respectively. Kinetic analysis of the reactions indicated that both substrates exhibited equivalent Vmax values, but that histamine's Km was ten-fold lower than that of alphamethylhistamine. these results indicate that alphamethylhistamine can be methylated as effectively as histamine, and may be a useful tool in further studies of HMT.
Original language | English |
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Pages (from-to) | 425-428 |
Number of pages | 4 |
Journal | Agents and Actions |
Volume | 11 |
Issue number | 5 |
DOIs | |
State | Published - Nov 1981 |
Externally published | Yes |