Alpha-methylhistamine methylation by histamine methyltransferase

Lindsay B. Hough, J. K. Khandelwal, Thomas W. Mittag

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Enzymatic methylation of histamine and analogues by histamine methyltransferase (HMT) was studied. Incubation of histamine or alpha-methylhistamine with HMT and S-adenosylmethionine was shown by GC-MS to yield tele-methyl and alpha-methyl-tele-methyl-histamine, respectively. Kinetic analysis of the reactions indicated that both substrates exhibited equivalent Vmax values, but that histamine's Km was ten-fold lower than that of alphamethylhistamine. these results indicate that alphamethylhistamine can be methylated as effectively as histamine, and may be a useful tool in further studies of HMT.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalAgents and Actions
Volume11
Issue number5
DOIs
StatePublished - Nov 1981
Externally publishedYes

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