TY - JOUR
T1 - Allelic imbalance in selected chromosomal regions in ovarian cancer
AU - Hansen, Lise Lotte
AU - Jensen, Lise Lind
AU - Dimitrakakis, Constantine
AU - Michalas, Stylianos
AU - Gilbert, Fred
AU - Barber, Hugh R.K.
AU - Overgaard, Jens
AU - Arzimanoglou, Iordanis I.
N1 - Funding Information:
The authors would like to thank E. Hein for her excellent technical assistance. This work was supported by The Danish Cancer Society, The Danish Research Council, Novo Nordisk Foundation, Kbm. B. Rasmussen and Wife Award, The Hugoton Foundation and the President of the Foundation, Joan Stout, Max and Inger Wørzner Memorial, Karen Krieger, Frits, Georg and Marie Cecilie Glud and A.P. Møller and Wife Foundations, and Captain Vassilis Constantacopoulos.
PY - 2002/11
Y1 - 2002/11
N2 - Ovarian cancer (OC) is often asymptomatic at the initial stage. When diagnosed, up to 75% of the patients present grade III or IV tumors with metastasis in nearby organs of the abdomen. Genetic imbalance is abundant in OC, and allelic loss (AL) of specific chromosomal regions is considered an early event. To establish association between genetic markers for early diagnosis/prognosis of OC, our target was to define narrow specific regions of AL. We analyzed 65 ovarian carcinomas by using 19 microsatellite markers located in three different chromosomes. First, a 7.6-Mb region containing the estrogen receptor (ESR1) and the tumor suppressor gene LATS1 was analyzed. Several chromosomal breakpoints flanking ESR1 affecting the region harboring LATS1 were found. Second, we found chromosomal breakpoints on 13q13.1∼q13.3 that defined two narrow regions flanking the BRCA2 locus. Third, our ovarian tumors exhibited a very high frequency of AL on 16q and chromosomal breakpoints defining two narrow regions within 16q22.2∼q24.3. In this article, we report three new polymorphic microsatellite markers and strong evidence of AL of narrow well-defined regions in hot spots on 6q, 13q, and 16q in ovarian tumors.
AB - Ovarian cancer (OC) is often asymptomatic at the initial stage. When diagnosed, up to 75% of the patients present grade III or IV tumors with metastasis in nearby organs of the abdomen. Genetic imbalance is abundant in OC, and allelic loss (AL) of specific chromosomal regions is considered an early event. To establish association between genetic markers for early diagnosis/prognosis of OC, our target was to define narrow specific regions of AL. We analyzed 65 ovarian carcinomas by using 19 microsatellite markers located in three different chromosomes. First, a 7.6-Mb region containing the estrogen receptor (ESR1) and the tumor suppressor gene LATS1 was analyzed. Several chromosomal breakpoints flanking ESR1 affecting the region harboring LATS1 were found. Second, we found chromosomal breakpoints on 13q13.1∼q13.3 that defined two narrow regions flanking the BRCA2 locus. Third, our ovarian tumors exhibited a very high frequency of AL on 16q and chromosomal breakpoints defining two narrow regions within 16q22.2∼q24.3. In this article, we report three new polymorphic microsatellite markers and strong evidence of AL of narrow well-defined regions in hot spots on 6q, 13q, and 16q in ovarian tumors.
UR - http://www.scopus.com/inward/record.url?scp=0036874254&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(02)00620-9
DO - 10.1016/S0165-4608(02)00620-9
M3 - Article
C2 - 12547149
AN - SCOPUS:0036874254
SN - 0165-4608
VL - 139
SP - 1
EP - 8
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -