ALKBH7 mediates necrosis via rewiring of glyoxal metabolism

Chaitanya A. Kulkarni, Sergiy M. Nadtochiy, Leslie Kennedy, Jimmy Zhang, Sophea Chhim, Hanan Alwaseem, Elizabeth Murphy, Dragony Fu, Paul S. Brookes

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Alkb homolog 7 (ALKBH7) is a mitochondrial α-ketoglutarate dioxygenase required for DNA alkylation-induced necrosis, but its function and substrates remain unclear. Herein, we show ALKBH7 regulates dialdehyde metabolism, which impacts the cardiac response to ischemia-reperfusion (IR) injury. Using a multi-omics approach, we find no evidence ALKBH7 functions as a prolyl-hydroxylase, but we do find Alkbh7-/- mice have elevated glyoxalase I (GLO-1), a dialdehyde detoxifying enzyme. Metabolic pathways related to the glycolytic by-product methylglyoxal (MGO) are rewired in Alkbh7-/- mice, along with elevated levels of MGO protein adducts. Despite greater glycative stress, hearts from Alkbh7-/- mice are protected against IR injury, in a manner blocked by GLO-1 inhibition. Integrating these observations, we propose ALKBH7 regulates glyoxal metabolism, and that protection against necrosis and cardiac IR injury bought on by ALKBH7 deficiency originates from the signaling response to elevated MGO stress.

Original languageEnglish
Article numbere58573
Pages (from-to)1-23
Number of pages23
JournaleLife
Volume9
DOIs
StatePublished - Aug 2020
Externally publishedYes

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