Alkaline extraction of cholecystokinin-immunoreactivity from rat brain

Steven W. Ryder; John Eng; Eugene Straus; Rosalyn S. Yalow

doi:10.1016/0006-291X(80)91289-9

Alkaline extraction of cholecystokinin-immunoreactivity from rat brain

Steven W. Ryder, John Eng, Eugene Straus, Rosalyn S. Yalow

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Alkaline aqueous extractants remove from rat brain 2 to 4 times the CCK-immunoreactivity that is removed by acidic or neutral aqueous extractants. The distribution among the various hormonal forms appears to be independent of the extractant: about 1 10 in the larger basic forms (CCK-33 and CCK-39); about 1 4 as the C-terminal dodecapeptide (CCK-12) and the remainder as the octapeptide (CCK-8). In contrast, alkaline and acidic aqueous solutions are equally efficient in extraction of enkephalin-immunoreactivity from the same tissues. We are presently unable to account for the very different efficiencies of the various extractants in removing CCK-immunoreactivity from brain.

Original language	English
Pages (from-to)	704-709
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	94
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)91289-9
State	Published - 30 May 1980
Externally published	Yes

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title = "Alkaline extraction of cholecystokinin-immunoreactivity from rat brain",

abstract = "Alkaline aqueous extractants remove from rat brain 2 to 4 times the CCK-immunoreactivity that is removed by acidic or neutral aqueous extractants. The distribution among the various hormonal forms appears to be independent of the extractant: about 1 10 in the larger basic forms (CCK-33 and CCK-39); about 1 4 as the C-terminal dodecapeptide (CCK-12) and the remainder as the octapeptide (CCK-8). In contrast, alkaline and acidic aqueous solutions are equally efficient in extraction of enkephalin-immunoreactivity from the same tissues. We are presently unable to account for the very different efficiencies of the various extractants in removing CCK-immunoreactivity from brain.",

author = "Ryder, {Steven W.} and John Eng and Eugene Straus and Yalow, {Rosalyn S.}",

note = "Funding Information: the extractant. ACKNOWLEDGEMENT This work was supported Veterans Administration.",

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T1 - Alkaline extraction of cholecystokinin-immunoreactivity from rat brain

AU - Ryder, Steven W.

AU - Eng, John

AU - Straus, Eugene

AU - Yalow, Rosalyn S.

N1 - Funding Information: the extractant. ACKNOWLEDGEMENT This work was supported Veterans Administration.

PY - 1980/5/30

Y1 - 1980/5/30

N2 - Alkaline aqueous extractants remove from rat brain 2 to 4 times the CCK-immunoreactivity that is removed by acidic or neutral aqueous extractants. The distribution among the various hormonal forms appears to be independent of the extractant: about 1 10 in the larger basic forms (CCK-33 and CCK-39); about 1 4 as the C-terminal dodecapeptide (CCK-12) and the remainder as the octapeptide (CCK-8). In contrast, alkaline and acidic aqueous solutions are equally efficient in extraction of enkephalin-immunoreactivity from the same tissues. We are presently unable to account for the very different efficiencies of the various extractants in removing CCK-immunoreactivity from brain.

AB - Alkaline aqueous extractants remove from rat brain 2 to 4 times the CCK-immunoreactivity that is removed by acidic or neutral aqueous extractants. The distribution among the various hormonal forms appears to be independent of the extractant: about 1 10 in the larger basic forms (CCK-33 and CCK-39); about 1 4 as the C-terminal dodecapeptide (CCK-12) and the remainder as the octapeptide (CCK-8). In contrast, alkaline and acidic aqueous solutions are equally efficient in extraction of enkephalin-immunoreactivity from the same tissues. We are presently unable to account for the very different efficiencies of the various extractants in removing CCK-immunoreactivity from brain.

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Alkaline extraction of cholecystokinin-immunoreactivity from rat brain

Abstract

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