Age-associated reduction of nuclear protein import in human fibroblasts

Gemma Pujol, Henrik Söderqvist, Aurelian Radu

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Age-dependent decreases in the protein concentrations of the nucleocytoplasmic transport factors karyopherin α2, CAS, and RanBP1 were found by comparing fibroblast cultures obtained from young, mature, and old human donors. Karyopherin β1 levels do not change with age and present very little variation among donors. The decrease in the concentration of transport factors is accompanied by a reduction in the protein import rate in fibroblasts from old donors, as detected by a change in the intracellular localization of a test transport substrate that shuttles between the cytoplasm and the nucleus. Measurements of concentrations of the same import factors in organs and tissues of old mice revealed a decrease of CAS in kidney, lung, and spleen. The import reduction in old age is expected to lead to impaired activity of proteins whose functions depend on timely import into the nuclei.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Ageing
  • Cell culture
  • Human fibroblast
  • Mouse organs
  • Nucleocytoplasmic transport

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