Affinity chromatography using enzymatically synthesized nucleotide-containing DNA binding polymers

Qun Wang, Robert J. Linhardt, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalBiotechnology Techniques
Volume13
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Affinity chromatography
  • DNA binding
  • Synthetic nucleosides

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