Aerosolized recombinant human lysozyme enhances the bactericidal effect of tobramycin in a hamster model of pseudomonas aeruginosainduced pneumonia

Tapan Bhavsar, Ming Liu, Xingjian Liu, Jerome Cantor

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Previous studies from this laboratory have shown that aerosolized recombinant human lysozyme (rhLZ) mitigates Pseudomonas aeruginosa (PA)-induced pneumonia. In the current investigation, our laboratory tested the hypothesis that aerosolized rhLZ can potentiate the effects of tobramycin (TBMN), thereby reducing the effective dose of this agent in the treatment of PA-induced pneumonia. Syrian hamsters were instilled intratracheally with PA, then exposed to an aerosol containing either 1% rhLZ, 3 μg TBMN, or a combination of both agents. In contrast to the initial studies with rhLZ, which involved 3 separate aerosol exposures, only a single treatment was used in the current investigation. Twenty-four hours after completion of the aerosol regimen, the following parameters were measured: (1) whole-lung colony-forming units (CFU), (2) total bronchoalveolar lavage fluid (BALF) CFU, (3) lung histopathology, and (4) total BALF neutrophils. The combination of rhLZ and TBMN significantly reduced whole-lung and BALF CFU, as well as the inflammatory index, compared to TBMN alone. Similar results were seen in vitro with regard to bactericidal activity. These findings provide a rationale for clinical testing of rhLZ as an adjunct to commercial antibiotic treatment.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalExperimental Lung Research
Volume37
Issue number9
DOIs
StatePublished - Nov 2011
Externally publishedYes

Keywords

  • lysozyme
  • pneumonia
  • pseudomonas
  • tobramycin

Fingerprint

Dive into the research topics of 'Aerosolized recombinant human lysozyme enhances the bactericidal effect of tobramycin in a hamster model of pseudomonas aeruginosainduced pneumonia'. Together they form a unique fingerprint.

Cite this