TY - JOUR
T1 - Advances in various techniques for isolation and purification of sterols
AU - Dikshit, Sneha
AU - Bubna, Sakshi
AU - Gupta, Anand
AU - Kumar, Piyush
N1 - Publisher Copyright:
© 2019, Association of Food Scientists & Technologists (India).
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Plants consist of triterpenoids such as phytosterols (PT) (C29H50O) with steroidal nuclei, including sitosterol, stigmasterol, brassicasterol and campesterol. They are hydrophobic but soluble in alcohol and other organic solvents and are isolated from industrial waste deodorizer distillates of various edible oil industries. They exist as free PT or their ester derivatives in soybean, rice, wheat, oat, cottonseed and corn fiber, and other cereals and grains. Conventional isolation techniques such as solvent extraction, distillation, evaporative fractionation, saponification and chemical esterification are employed for isolation and purification of PT. The present article reviews the various advanced separation techniques like solvent crystallization, supercritical fluid extraction, high speed counter-current chromatography and enzymatic process as strategic methods to isolate and purify sterols.
AB - Plants consist of triterpenoids such as phytosterols (PT) (C29H50O) with steroidal nuclei, including sitosterol, stigmasterol, brassicasterol and campesterol. They are hydrophobic but soluble in alcohol and other organic solvents and are isolated from industrial waste deodorizer distillates of various edible oil industries. They exist as free PT or their ester derivatives in soybean, rice, wheat, oat, cottonseed and corn fiber, and other cereals and grains. Conventional isolation techniques such as solvent extraction, distillation, evaporative fractionation, saponification and chemical esterification are employed for isolation and purification of PT. The present article reviews the various advanced separation techniques like solvent crystallization, supercritical fluid extraction, high speed counter-current chromatography and enzymatic process as strategic methods to isolate and purify sterols.
KW - Chromatography
KW - Counter-current chromatography
KW - Crystallization
KW - Phytosterols
KW - Supercritical fluid extraction
KW - Tocopherols
UR - http://www.scopus.com/inward/record.url?scp=85076929330&partnerID=8YFLogxK
U2 - 10.1007/s13197-019-04209-3
DO - 10.1007/s13197-019-04209-3
M3 - Review article
AN - SCOPUS:85076929330
SN - 0022-1155
VL - 57
SP - 2393
EP - 2403
JO - Journal of Food Science and Technology
JF - Journal of Food Science and Technology
IS - 7
ER -