Adoptive transfer of cells sensitized in vitro into mice in a skin allograft assay

M. Margaret Kemeny, Paul H. Sugarbaker

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In these experiments we investigated the ability of adoptively transferred in vitro-sensitized cells to cause an accelerated rejection of skin allografts. The survival of B10.BR or B10.D2 skin grafts on B6AF1 mice was measured. It was determined that 5 × 107 in vitro-sensitized cells were required for a consistent accelerated skin allograft rejection. Attempts to optimize sensitization using syngeneic mouse serum were unsuccessful. In vitro-sensitized lymphocytes were specific in their activity toward skin allografts, but were nonspecific in their lysis of tumor targets. Inadvertant transfer of alloantigen with in vitro-sensitized cells was not responsible for accelerated graft rejection. This work demonstrates that cells sensitized in vitro can cause specific accelerated skin allograft rejection in normal mice.

Original languageEnglish
Pages (from-to)197-206
Number of pages10
JournalCellular Immunology
Issue number1
StatePublished - Feb 1982
Externally publishedYes


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