Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies

Peter H. Goff, Dirk Eggink, Christopher W. Seibert, Rong Hai, Luis Martínez-Gil, Florian Krammer, Peter Palese

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.

Original languageEnglish
Article numbere79194
JournalPLoS ONE
Issue number11
StatePublished - 6 Nov 2013


Dive into the research topics of 'Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies'. Together they form a unique fingerprint.

Cite this