Adherent culture of CDI33 + cells in glioblastomas and expression of ADLH1

Objective To investigate the influence of adherent culture on the acquisition of CD133⁺ cells in glioblastomas and the expressions of acetaldehyde dehydrogenase 1 (ALDH1) in the undifferentiated and differentiated cells. Methods Adherent culture was performed with immunomagnetic bead technique in 7 glioblastoma samples to gain CD133⁺ cells. The cell differentiation was induced via serum medium culture. Immunocytochemistry staining was used to detect the expressions of ALDH1 and Sox2 in the undifferentiated and differentiated cells. Results CD133⁺ cells were obtained in 6 samples and typical tumor spheres were observed. Positive expressions of ALDH1 and Sox2 were detected in the undifferentiated cells and co-localization was found. No expressions of those two markers in the differentiated cells were observed. Conclusion CD133⁺ cells can be efficiently obtained via adherent culture method. ALDH1 expression appears only in the undifferentiated cells, which can be a new marker for glioma stem cells.