TY - JOUR
T1 - Adenylate cyclase enhancing factor from rat osteosarcoma cytosol
AU - Egan, J. J.
AU - Majeska, R. J.
AU - Rodan, G. A.
N1 - Funding Information:
This study was supported by U.S.P.H.S. grants We thank Ms. S. Pearson for typing the manuscript.
PY - 1978/1/13
Y1 - 1978/1/13
N2 - A cytosol factor from a transplantable rat osteosarcoma stimulates the adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) activity of partially purified membranes 1.5 fold at pH 7.6 and over 5 fold at pH 6.5. This effect can also be seen at maximum Gpp(NH) stimulation of the enzyme. The cytosol factor is non-dialyzable, ultrafiltrable through a 100,000 dalton exclusion membrane, heat labile and trypsin digestible. The stimulation is immediate, is independent of Ca2+, exhibits sygmoidal concentration dependency and is enhanced by GTP. The factor did not bind GTP. The stimulatory activity was fully recovered in two Sephadex G-100 fractions of approximate molecular weights of 55,000 and 29,500. Unlike the starting material the fractions were not stable to freeze-thawing or lyophilization. A similar factor could not be found in embryonic bone, nor did the osteosarcoma factor affect bone adenylate cyclase.
AB - A cytosol factor from a transplantable rat osteosarcoma stimulates the adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) activity of partially purified membranes 1.5 fold at pH 7.6 and over 5 fold at pH 6.5. This effect can also be seen at maximum Gpp(NH) stimulation of the enzyme. The cytosol factor is non-dialyzable, ultrafiltrable through a 100,000 dalton exclusion membrane, heat labile and trypsin digestible. The stimulation is immediate, is independent of Ca2+, exhibits sygmoidal concentration dependency and is enhanced by GTP. The factor did not bind GTP. The stimulatory activity was fully recovered in two Sephadex G-100 fractions of approximate molecular weights of 55,000 and 29,500. Unlike the starting material the fractions were not stable to freeze-thawing or lyophilization. A similar factor could not be found in embryonic bone, nor did the osteosarcoma factor affect bone adenylate cyclase.
UR - http://www.scopus.com/inward/record.url?scp=0017839198&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(78)91120-8
DO - 10.1016/0006-291X(78)91120-8
M3 - Article
C2 - 414750
AN - SCOPUS:0017839198
SN - 0006-291X
VL - 80
SP - 176
EP - 182
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -