TY - JOUR
T1 - Additional evidence for an eight-transmembrane-domain topology for caenorhabditis elegans and human presenilins
AU - Li, Xiajun
AU - Greenwald, Iva
PY - 1998/6/9
Y1 - 1998/6/9
N2 - Presenilins have been implicated in the genesis of Alzheimer's disease and in facilitating LIN-12/Notch activity during development. All presenilins have multiple hydrophobic regions that could theoretically span a membrane, and a description of the membrane topology is a crucial step toward deducing the mechanism of presenilin function. Previously, we proposed an eight- transmembrane-domain model for presenilin, based on studies of the Caenorhabditis elegans SEL-12 presenilin. Here, we describe experiments that support the view that two of the hydrophobic regions of SEL-12 function as the seventh and eighth transmembrane domains. Furthermore, we have shown that human presenilin I behaves like SEL-12 presenilin when analyzed by our methods. Our results provide additional experimental support for the eight- transmembrane-domain model of presenilin topology.
AB - Presenilins have been implicated in the genesis of Alzheimer's disease and in facilitating LIN-12/Notch activity during development. All presenilins have multiple hydrophobic regions that could theoretically span a membrane, and a description of the membrane topology is a crucial step toward deducing the mechanism of presenilin function. Previously, we proposed an eight- transmembrane-domain model for presenilin, based on studies of the Caenorhabditis elegans SEL-12 presenilin. Here, we describe experiments that support the view that two of the hydrophobic regions of SEL-12 function as the seventh and eighth transmembrane domains. Furthermore, we have shown that human presenilin I behaves like SEL-12 presenilin when analyzed by our methods. Our results provide additional experimental support for the eight- transmembrane-domain model of presenilin topology.
UR - http://www.scopus.com/inward/record.url?scp=0032499750&partnerID=8YFLogxK
U2 - 10.1073/pnas.95.12.7109
DO - 10.1073/pnas.95.12.7109
M3 - Article
C2 - 9618547
AN - SCOPUS:0032499750
SN - 0027-8424
VL - 95
SP - 7109
EP - 7114
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -