Additional evidence for an eight-transmembrane-domain topology for caenorhabditis elegans and human presenilins

Xiajun Li, Iva Greenwald

Research output: Contribution to journalArticlepeer-review

142 Scopus citations

Abstract

Presenilins have been implicated in the genesis of Alzheimer's disease and in facilitating LIN-12/Notch activity during development. All presenilins have multiple hydrophobic regions that could theoretically span a membrane, and a description of the membrane topology is a crucial step toward deducing the mechanism of presenilin function. Previously, we proposed an eight- transmembrane-domain model for presenilin, based on studies of the Caenorhabditis elegans SEL-12 presenilin. Here, we describe experiments that support the view that two of the hydrophobic regions of SEL-12 function as the seventh and eighth transmembrane domains. Furthermore, we have shown that human presenilin I behaves like SEL-12 presenilin when analyzed by our methods. Our results provide additional experimental support for the eight- transmembrane-domain model of presenilin topology.

Original languageEnglish
Pages (from-to)7109-7114
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number12
DOIs
StatePublished - 9 Jun 1998
Externally publishedYes

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