Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3

Hong Liang Zhang; Yan Dong Tang; Chun Xiao Liu; Li Run Xiang; Wen Li Zhang; Chao Liang Leng; Qian Wang; Tong Qing An; Jin Mei Peng; Zhi Jun Tian; Xue Hui Cai

doi:10.1016/j.vetmic.2018.06.021

Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3

Hong Liang Zhang
, Yan Dong Tang
, Chun Xiao Liu
, Li Run Xiang
, Wen Li Zhang
, Chao Liang Leng
, Qian Wang
, Tong Qing An
, Jin Mei Peng
, Zhi Jun Tian
, Xue Hui Cai

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.

Original language	English
Pages (from-to)	46-54
Number of pages	9
Journal	Veterinary Microbiology
Volume	222
DOIs	https://doi.org/10.1016/j.vetmic.2018.06.021
State	Published - Aug 2018
Externally published	Yes

Keywords

GP2a and GP3
MY-376
Marc-145-unadaptive
Tropism
Variation

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10.1016/j.vetmic.2018.06.021

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@article{abd0b3a3242f4e8b9e36d060787634bb,

title = "Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3",

abstract = "The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.",

keywords = "GP2a and GP3, MY-376, Marc-145-unadaptive, Tropism, Variation",

author = "Zhang, \{Hong Liang\} and Tang, \{Yan Dong\} and Liu, \{Chun Xiao\} and Xiang, \{Li Run\} and Zhang, \{Wen Li\} and Leng, \{Chao Liang\} and Qian Wang and An, \{Tong Qing\} and Peng, \{Jin Mei\} and Tian, \{Zhi Jun\} and Cai, \{Xue Hui\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = aug,

doi = "10.1016/j.vetmic.2018.06.021",

language = "English",

volume = "222",

pages = "46--54",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3

AU - Zhang, Hong Liang

AU - Tang, Yan Dong

AU - Liu, Chun Xiao

AU - Xiang, Li Run

AU - Zhang, Wen Li

AU - Leng, Chao Liang

AU - Wang, Qian

AU - An, Tong Qing

AU - Peng, Jin Mei

AU - Tian, Zhi Jun

AU - Cai, Xue Hui

PY - 2018/8

Y1 - 2018/8

N2 - The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.

AB - The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.

KW - GP2a and GP3

KW - MY-376

KW - Marc-145-unadaptive

KW - Tropism

KW - Variation

UR - https://www.scopus.com/pages/publications/85049426215

U2 - 10.1016/j.vetmic.2018.06.021

DO - 10.1016/j.vetmic.2018.06.021

M3 - Article

C2 - 30080672

AN - SCOPUS:85049426215

SN - 0378-1135

VL - 222

SP - 46

EP - 54

JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

Adaptions of field PRRSVs in Marc-145 cells were determined by variations in the minor envelope proteins GP2a-GP3

Abstract

Keywords

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