TY - JOUR
T1 - Acute ethanol ingestion produces dose-dependent effects on motor behavior in the honey bee (Apis mellifera)
AU - Maze, Ian S.
AU - Wright, Geraldine A.
AU - Mustard, Julie A.
N1 - Funding Information:
The authors wish to thank Sue Cobey for maintaining the honey bee colonies, Shanza Mujeeb for help with the hemolymph ethanol assays, and Brian Smith for support and access to facilities. We are also grateful to Roger Santer and Mitch Thomson for helpful comments on the manuscript. This work was supported by NIH (NIDA) Grant DA017694 to JAM; GAW was supported in part by NSF agreement no. 0112050 awarded to the Mathematical Biosciences Institute (OSU) and in part by NIH (NCRR) grant awarded to B.H. Smith (9 R01 RR1466); and the research was also supported by Ohio State University College of Biological Sciences Dean's Undergraduate Research Awards to ISM.
PY - 2006/11
Y1 - 2006/11
N2 - Ethanol consumption produces characteristic behavioral states in animals that include sedation, disorientation, and disruption of motor function. Using individual honey bees, we assessed the effects of ethanol ingestion on motor function via continuous observations of their behavior. Consumption of 1 M sucrose solutions containing a range of ethanol doses led to hemolymph ethanol levels of approximately 40-100 mM. Using ethanol doses in this range, we observed time and dose-dependent effects of ethanol on the percent of time our subjects spent walking, stopped, or upside down, and on the duration and frequency of bouts of behavior. The effects on grooming and flying behavior were more complex. Behavioral recovery from ethanol treatment was both time and ethanol dose dependent, occurring between 12 and 24 h post-ingestion for low doses and at 24-48 h for higher doses. Furthermore, the amount of ethanol measured in honey bee hemolymph appeared to correlate with recovery. We predict that the honey bee will prove to be an excellent model system for studying the influence of ethanol on the neural mechanisms underlying behavior.
AB - Ethanol consumption produces characteristic behavioral states in animals that include sedation, disorientation, and disruption of motor function. Using individual honey bees, we assessed the effects of ethanol ingestion on motor function via continuous observations of their behavior. Consumption of 1 M sucrose solutions containing a range of ethanol doses led to hemolymph ethanol levels of approximately 40-100 mM. Using ethanol doses in this range, we observed time and dose-dependent effects of ethanol on the percent of time our subjects spent walking, stopped, or upside down, and on the duration and frequency of bouts of behavior. The effects on grooming and flying behavior were more complex. Behavioral recovery from ethanol treatment was both time and ethanol dose dependent, occurring between 12 and 24 h post-ingestion for low doses and at 24-48 h for higher doses. Furthermore, the amount of ethanol measured in honey bee hemolymph appeared to correlate with recovery. We predict that the honey bee will prove to be an excellent model system for studying the influence of ethanol on the neural mechanisms underlying behavior.
KW - Alcohol
KW - Central pattern generator
KW - Grooming
KW - Invertebrate
KW - Locomotion
UR - http://www.scopus.com/inward/record.url?scp=33750972874&partnerID=8YFLogxK
U2 - 10.1016/j.jinsphys.2006.09.006
DO - 10.1016/j.jinsphys.2006.09.006
M3 - Article
C2 - 17070538
AN - SCOPUS:33750972874
SN - 0022-1910
VL - 52
SP - 1243
EP - 1253
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
IS - 11-12
ER -