Activation of p42/44 mitogen-activated protein kinase pathway in long-term potentiation induced by nicotine in hippocampal CA1 region in rats

J. Wang; Y. B. Chen; X. N. Zhu; R. Z. Chen

Activation of p42/44 mitogen-activated protein kinase pathway in long-term potentiation induced by nicotine in hippocampal CA1 region in rats

J. Wang
, Y. B. Chen
, X. N. Zhu
, R. Z. Chen

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

AIM: To investigate the relationship between activation of p42/44 mitogen-activated protein kinase. (MAPK) pathway and hippocampal long term potentiation (LTP) induced by nicotine in area CA1. METHODS: Extracellular recording of population spike (PS) was performed within the pyramidal cell layer of hippocampal area CA1 in vitro; Western blot analysis was employed to detect the active phosphorylated state and the total protein expression of p42/44 MAPK. RESULTS: PD98059 concentration-dependently (25 μmol/L, 50 μmol/L) attenuated the induction of LTP induced by nicotine 10 μmol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. CONCLUSION: Activation of p42/44 MAPK pathway is required for hippocampal LTP induced by nicotine.

Original language	English
Pages (from-to)	685-690
Number of pages	6
Journal	Acta Pharmacologica Sinica
Volume	22
Issue number	8
State	Published - 2001
Externally published	Yes

Keywords

Hippocampus
Long-term potentiation
Mitogen-activated protein kinase
Nicotine
Western blotting

Cite this

@article{3a96a0c0fff2429288889acd2f2c808d,

title = "Activation of p42/44 mitogen-activated protein kinase pathway in long-term potentiation induced by nicotine in hippocampal CA1 region in rats",

abstract = "AIM: To investigate the relationship between activation of p42/44 mitogen-activated protein kinase. (MAPK) pathway and hippocampal long term potentiation (LTP) induced by nicotine in area CA1. METHODS: Extracellular recording of population spike (PS) was performed within the pyramidal cell layer of hippocampal area CA1 in vitro; Western blot analysis was employed to detect the active phosphorylated state and the total protein expression of p42/44 MAPK. RESULTS: PD98059 concentration-dependently (25 μmol/L, 50 μmol/L) attenuated the induction of LTP induced by nicotine 10 μmol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. CONCLUSION: Activation of p42/44 MAPK pathway is required for hippocampal LTP induced by nicotine.",

keywords = "Hippocampus, Long-term potentiation, Mitogen-activated protein kinase, Nicotine, Western blotting",

author = "J. Wang and Chen, \{Y. B.\} and Zhu, \{X. N.\} and Chen, \{R. Z.\}",

year = "2001",

language = "English",

volume = "22",

pages = "685--690",

journal = "Acta Pharmacologica Sinica",

issn = "0253-9756",

publisher = "Springer Nature",

number = "8",

}

TY - JOUR

T1 - Activation of p42/44 mitogen-activated protein kinase pathway in long-term potentiation induced by nicotine in hippocampal CA1 region in rats

AU - Wang, J.

AU - Chen, Y. B.

AU - Zhu, X. N.

AU - Chen, R. Z.

PY - 2001

Y1 - 2001

N2 - AIM: To investigate the relationship between activation of p42/44 mitogen-activated protein kinase. (MAPK) pathway and hippocampal long term potentiation (LTP) induced by nicotine in area CA1. METHODS: Extracellular recording of population spike (PS) was performed within the pyramidal cell layer of hippocampal area CA1 in vitro; Western blot analysis was employed to detect the active phosphorylated state and the total protein expression of p42/44 MAPK. RESULTS: PD98059 concentration-dependently (25 μmol/L, 50 μmol/L) attenuated the induction of LTP induced by nicotine 10 μmol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. CONCLUSION: Activation of p42/44 MAPK pathway is required for hippocampal LTP induced by nicotine.

AB - AIM: To investigate the relationship between activation of p42/44 mitogen-activated protein kinase. (MAPK) pathway and hippocampal long term potentiation (LTP) induced by nicotine in area CA1. METHODS: Extracellular recording of population spike (PS) was performed within the pyramidal cell layer of hippocampal area CA1 in vitro; Western blot analysis was employed to detect the active phosphorylated state and the total protein expression of p42/44 MAPK. RESULTS: PD98059 concentration-dependently (25 μmol/L, 50 μmol/L) attenuated the induction of LTP induced by nicotine 10 μmol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. CONCLUSION: Activation of p42/44 MAPK pathway is required for hippocampal LTP induced by nicotine.

KW - Hippocampus

KW - Long-term potentiation

KW - Mitogen-activated protein kinase

KW - Nicotine

KW - Western blotting

UR - https://www.scopus.com/pages/publications/0034887338

M3 - Article

C2 - 11749838

AN - SCOPUS:0034887338

SN - 0253-9756

VL - 22

SP - 685

EP - 690

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

IS - 8

ER -

Activation of p42/44 mitogen-activated protein kinase pathway in long-term potentiation induced by nicotine in hippocampal CA1 region in rats

Abstract

Keywords

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