Activation of MKK3/6, SAPK, and ATF-2/c-jun in ACL fibroblasts grown in 3 dimension collagen gels in response to application of cyclic strain

Vonda Wright, Erik Attia, Krista Bohnert, Haydee Brown, Madhu Bhargava, Jo A. Hannafin

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Signal transduction pathways involved in response to cyclic tensile strain and strain deprivation in anterior cruciate ligament (ACL) fibroblasts grown in 3D collagen gels were investigated. Application of cyclic tensile strain resulted in significant activation (phosphorylation) of MKK3/6, SAPK and their downstream target transcription factors, ATF-2 and c-jun, while strain deprivation resulted in a decrease in these kinases and transcription factors. These data suggest that ACL fibroblasts cultured in 3D collagen gels respond to the mechanical environment and provide a useful system for determination of the molecular mechanisms involved in the regulation of proliferation and matrix turnover by mechanical load.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalJournal of Orthopaedic Research
Volume29
Issue number3
DOIs
StatePublished - Mar 2011
Externally publishedYes

Keywords

  • ACL fibroblast
  • ATF-2
  • MKK3/6
  • SAPK
  • c-Jun
  • cyclic strain

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