Activation of human NK cells by malaria-infected red blood cells.

Amir Horowitz; Eleanor M. Riley

doi:10.1007/978-1-60761-362-6_29

Activation of human NK cells by malaria-infected red blood cells.

Amir Horowitz, Eleanor M. Riley

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.

Original language	English
Pages (from-to)	429-446
Number of pages	18
Journal	Methods in Molecular Biology
Volume	612
DOIs	https://doi.org/10.1007/978-1-60761-362-6_29
State	Published - 2010
Externally published	Yes

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title = "Activation of human NK cells by malaria-infected red blood cells.",

abstract = "This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.",

author = "Amir Horowitz and Riley, \{Eleanor M.\}",

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journal = "Methods in Molecular Biology",

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AU - Horowitz, Amir

AU - Riley, Eleanor M.

PY - 2010

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N2 - This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.

AB - This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.

UR - https://www.scopus.com/pages/publications/77449154111

U2 - 10.1007/978-1-60761-362-6_29

DO - 10.1007/978-1-60761-362-6_29

M3 - Article

C2 - 20033658

AN - SCOPUS:77449154111

SN - 1064-3745

VL - 612

SP - 429

EP - 446

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Activation of human NK cells by malaria-infected red blood cells.

Abstract

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