TY - JOUR
T1 - Acetylation of sphingosine bases and long-chain amines by cell-free preparations of Hansenula ciferri
AU - Barenholz, Yechezkel
AU - Gatt, Shimon
N1 - Funding Information:
Acknowledgements: This work is part of a Ph. D. thesis to be submitted to the Senate of the Hebrew University. It was supported by a U. S. Public Health Research Service Grant (NBO2967).W e wish to thank Dr. Y. Bartana for many valuable discussions in the course of this investigation.
PY - 1969/6/6
Y1 - 1969/6/6
N2 - A cell-free preparation of the yeast, Hansenula ciferri catalyzed the transfer of the acetyl group of acetyl coenzyme-A to the sphinosine bases at both their amino and hydroxyl groups. The enzyme also transferred acetyl groups to the hydroxyls of the N-acetylated sphingosine bases as well as to the amino groups of primary amines of ten or more carbon atoms. A mixture of acetate, ATP and coenzyme-A could be employed instead of acetyl CoA. The reaction had an optimal pH at about 7.8 and was inhibited by free coenzyme-A.
AB - A cell-free preparation of the yeast, Hansenula ciferri catalyzed the transfer of the acetyl group of acetyl coenzyme-A to the sphinosine bases at both their amino and hydroxyl groups. The enzyme also transferred acetyl groups to the hydroxyls of the N-acetylated sphingosine bases as well as to the amino groups of primary amines of ten or more carbon atoms. A mixture of acetate, ATP and coenzyme-A could be employed instead of acetyl CoA. The reaction had an optimal pH at about 7.8 and was inhibited by free coenzyme-A.
UR - http://www.scopus.com/inward/record.url?scp=0014665752&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(69)90458-6
DO - 10.1016/0006-291X(69)90458-6
M3 - Article
C2 - 5794085
AN - SCOPUS:0014665752
SN - 0006-291X
VL - 35
SP - 676
EP - 680
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -