Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure

I. M. Shapiro; M. H. Moar; S. Ohno; G. Klein

doi:10.1016/0014-4827(78)90296-3

Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure

I. M. Shapiro
, M. H. Moar
, S. Ohno
, G. Klein

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Acetic acid (pH 2.0) treatment denatures chromosomal DNA in situ. Following in situ hybridization cellular and chromosomal morphology is well preserved. For AT-rich DNA sequences the final efficiency of the subsequent hybridization reaction is comparable to the routinely employed NAOH denaturation method, and although there is some discrimination against denaturation of high GC-rich DNA sequences it is less marked than with HCl.

Original language	English
Pages (from-to)	411-414
Number of pages	4
Journal	Experimental Cell Research
Volume	115
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(78)90296-3
State	Published - Sep 1978
Externally published	Yes

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@article{3c483a5daff3473ba48dc9aa24922448,

title = "Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure",

abstract = "Acetic acid (pH 2.0) treatment denatures chromosomal DNA in situ. Following in situ hybridization cellular and chromosomal morphology is well preserved. For AT-rich DNA sequences the final efficiency of the subsequent hybridization reaction is comparable to the routinely employed NAOH denaturation method, and although there is some discrimination against denaturation of high GC-rich DNA sequences it is less marked than with HCl.",

author = "Shapiro, \{I. M.\} and Moar, \{M. H.\} and S. Ohno and G. Klein",

note = "Funding Information: We are indebted to Dr Meir Wilchek of the Weizmann Institute of Science, Rehovot, Israel, for the amino acid hydrolysis and determination of the amino acid reacted with bromocolchicine. [3HjBromocolchicine was synthesized by Dr D. Atlas of the Hebrew University of Jerusalem. This work was supported by a grant from the Caisse G\&r\&ale d{\textquoteright}Epargne et de Retraite (Fonds Cancerologique). Funding Information: We are grateful to Drs H. Koprowski and Z. Step-lewski who have madea nd sent us the humanm ouse somaticc ell hybrid. Mouse satelliteD NA was a generous gift from Mr I. F. Purdom (Edinburgh, Scotland). The work was supportedb y Grant Number 5ROl CA 14054-05a,w ardedb y the NCI, DEHW. M. H. M. was an EMBO Fellowship recipient.",

year = "1978",

month = sep,

doi = "10.1016/0014-4827(78)90296-3",

language = "English",

volume = "115",

pages = "411--414",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure

AU - Shapiro, I. M.

AU - Moar, M. H.

AU - Ohno, S.

AU - Klein, G.

N1 - Funding Information: We are indebted to Dr Meir Wilchek of the Weizmann Institute of Science, Rehovot, Israel, for the amino acid hydrolysis and determination of the amino acid reacted with bromocolchicine. [3HjBromocolchicine was synthesized by Dr D. Atlas of the Hebrew University of Jerusalem. This work was supported by a grant from the Caisse G&r&ale d’Epargne et de Retraite (Fonds Cancerologique). Funding Information: We are grateful to Drs H. Koprowski and Z. Step-lewski who have madea nd sent us the humanm ouse somaticc ell hybrid. Mouse satelliteD NA was a generous gift from Mr I. F. Purdom (Edinburgh, Scotland). The work was supportedb y Grant Number 5ROl CA 14054-05a,w ardedb y the NCI, DEHW. M. H. M. was an EMBO Fellowship recipient.

PY - 1978/9

Y1 - 1978/9

N2 - Acetic acid (pH 2.0) treatment denatures chromosomal DNA in situ. Following in situ hybridization cellular and chromosomal morphology is well preserved. For AT-rich DNA sequences the final efficiency of the subsequent hybridization reaction is comparable to the routinely employed NAOH denaturation method, and although there is some discrimination against denaturation of high GC-rich DNA sequences it is less marked than with HCl.

AB - Acetic acid (pH 2.0) treatment denatures chromosomal DNA in situ. Following in situ hybridization cellular and chromosomal morphology is well preserved. For AT-rich DNA sequences the final efficiency of the subsequent hybridization reaction is comparable to the routinely employed NAOH denaturation method, and although there is some discrimination against denaturation of high GC-rich DNA sequences it is less marked than with HCl.

UR - https://www.scopus.com/pages/publications/0018159282

U2 - 10.1016/0014-4827(78)90296-3

DO - 10.1016/0014-4827(78)90296-3

M3 - Article

C2 - 689097

AN - SCOPUS:0018159282

SN - 0014-4827

VL - 115

SP - 411

EP - 414

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure

Abstract

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