Abnormal cytolytic activity of lymphocyte function-associated antigen-1-deficient human cytolytic T lymphocyte clones

S. J. Mentzer, B. E. Bierer, D. C. Anderson, T. A. Springer, S. J. Burakoff

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15 Scopus citations

Abstract

The involvement of the lymphocyte function-associated antigen-1 (LFA-1) membrane molecule in cytolytic T lymphocyte (CTL) interactions with lymphoid target cells was investigated using CTL clones derived from two patients with a heritable deficiency of LFA-1. LFA-1 surface expression on the CTL clones was 1% of the normal level of LFA-1, unchanged with prolonged culture, and identical on 14 different CTL clones. The function of the LFA-1 molecule was addressed using the LFA-1-deficient CTL clones and LFA-1-deficient lymphoid target cells. The lytic activity of the LFA-1-deficient CTL clones was 43% of control when tested against a target cell line expressing normal levels of LFA-1 and <10% of control when tested against an LFA-1-deficient target cell line. These results demonstrate a direct involvement of LFA-1 in CTL-mediated cytolysis and suggest a more general dependence of LFA-1 in lymphoid cell-cell interactions.

Original languageEnglish
Pages (from-to)1387-1391
Number of pages5
JournalJournal of Clinical Investigation
Volume78
Issue number5
DOIs
StatePublished - 1986

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