Aberrant EVI1 expression in acute myeloid leukemias associated with the t(3;8)(q26;q24)

Patrick A. Lennon, Lynne V. Abruzzo, L. Jeffrey Medeiros, Candy Cromwell, Xiang Zhang, Cameron C. Yin, Steven M. Kornblau, Marina Konopieva, Pei Lin

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

EVI is a proto-oncogene that is activated in acute myeloid leukemia with chromosomal rearrangements that map to chromosome 3q26. We previously reported the clinicopathologic features of five cases of acute myeloid leukemia carrying t(3;8)(q26;q24). Using fluorescence in situ hybridization analysis, we demonstrate in the current study that the breakpoint on chromosome 3 is at EVI1/MDS1, and the breakpoint on chromosome 8 is just distal to the PVT1 oncogene homolog, a C-MYC activator in mice. The breakpoint on chromosome 8 was detected between the components of the LSI MYC dual-color break-apart rearrangement probe. Reverse-transcriptase polymerase chain reaction assay showed expression of EVI1 in all four cases analyzed, and DNA sequence analysis confirmed the findings. Reverse transcriptase polymerase chain reaction assay also demonstrated the expression of PVT1 and C-MYC in all four cases assessed. Western blot analysis detected EVI1 in one case analyzed. We conclude that the t(3;8)(q26;q24) results in deregulated EVI1 expression, similar to other balanced or unbalanced chromosomal translocations involving chromosome 3q26.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume177
Issue number1
DOIs
StatePublished - Aug 2007
Externally publishedYes

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