@inbook{025e1080d0a045d2bd5c3d95131f47bc,
title = "AAV vectors for efficient gene delivery to rodent hearts",
abstract = "Currently, gene therapy is one of the most promising fields in biomedicine, with great therapeutic potential for an array of inherited and acquired diseases. Adeno-associated viral (AAV) vectors have emerged as promising tools to deliver selectively a therapeutic payload to target organs, including the heart. In this chapter, we describe the production and quality control of recombinant AAV (rAAV) vectors of the serotype 9, the most cardiotropic AAV serotype when delivered systemically in rodents. We also describe the systemic administration of rAAV vectors and the local delivery of rAAV vectors by direct intramyocardial injection. Taken together, the methods described in this chapter will allow the reader to deliver efficiently therapeutic genes to the rodent heart, both globally and regionally.",
keywords = "AAV characterization, AAV delivery, AAV production, AAV9, Adeno-associated virus, Mouse, Rat",
author = "Estrella Lopez-Gordo and Erik Kohlbrenner and Katz, \{Michael G.\} and Thomas Weber",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9139-6\_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "311--332",
booktitle = "Methods in Molecular Biology",
}