A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21

Fergus J. Couch; Lucio H. Castilla; Junzhe Xu; Kenneth J. Abel; Piri Welcsh; Stephanie E. King; Linghua Wong; Peggy P. Ho; Sofia Merajver; Lawrence C. Brody; Guiying Yin; Steve T. Hayes; Linn M. Gieser; Wendy L. Flejter; Thomas W. Glover; Lori S. Friedman; Eric D. Lynch; Jose E. Meza; Mary Claire King; David J. Law; Larry Deaven; Anne M. Bowcock; Francis S. Collins; Barbara L. Weber; Settara C. Chandrasekharappa

doi:10.1016/0888-7543(95)80134-8

A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21

Fergus J. Couch
, Lucio H. Castilla
, Junzhe Xu
, Kenneth J. Abel
, Piri Welcsh
, Stephanie E. King
, Linghua Wong
, Peggy P. Ho
, Sofia Merajver
, Lawrence C. Brody
, Guiying Yin
, Steve T. Hayes
, Linn M. Gieser
, Wendy L. Flejter
, Thomas W. Glover
, Lori S. Friedman
, Eric D. Lynch
, Jose E. Meza
, Mary Claire King
, David J. Law

Larry Deaven, Anne M. Bowcock, Francis S. Collins, Barbara L. Weber, Settara C. Chandrasekharappa

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the interval has also been converted to a cosmid contig. Twenty-three PCR-based sequence-tagged sites (STSs) were mapped to these contigs, thereby confirming the order and overlap of individual clones. This complex physical map of the BRCA1 region was used to isolate genes by a number of gene identification techniques and to generate transcript maps of the region, as presented in the three accompanying manuscripts of Brody et al. (1995), Osborne-Lawrence et al. (1995), and Friedman et al. (1995).

Original language	English
Pages (from-to)	264-273
Number of pages	10
Journal	Genomics
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0888-7543(95)80134-8
State	Published - 1 Jan 1995
Externally published	Yes

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Couch, F. J., Castilla, L. H., Xu, J., Abel, K. J., Welcsh, P., King, S. E., Wong, L., Ho, P. P., Merajver, S., Brody, L. C., Yin, G., Hayes, S. T., Gieser, L. M., Flejter, W. L., Glover, T. W., Friedman, L. S., Lynch, E. D., Meza, J. E., King, M. C., ... Chandrasekharappa, S. C. (1995). A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21. Genomics, 25(1), 264-273. https://doi.org/10.1016/0888-7543(95)80134-8

@article{17749bdb398643969ea26e6a1b1e9ad9,

title = "A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21",

abstract = "A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the interval has also been converted to a cosmid contig. Twenty-three PCR-based sequence-tagged sites (STSs) were mapped to these contigs, thereby confirming the order and overlap of individual clones. This complex physical map of the BRCA1 region was used to isolate genes by a number of gene identification techniques and to generate transcript maps of the region, as presented in the three accompanying manuscripts of Brody et al. (1995), Osborne-Lawrence et al. (1995), and Friedman et al. (1995).",

author = "Couch, \{Fergus J.\} and Castilla, \{Lucio H.\} and Junzhe Xu and Abel, \{Kenneth J.\} and Piri Welcsh and King, \{Stephanie E.\} and Linghua Wong and Ho, \{Peggy P.\} and Sofia Merajver and Brody, \{Lawrence C.\} and Guiying Yin and Hayes, \{Steve T.\} and Gieser, \{Linn M.\} and Flejter, \{Wendy L.\} and Glover, \{Thomas W.\} and Friedman, \{Lori S.\} and Lynch, \{Eric D.\} and Meza, \{Jose E.\} and King, \{Mary Claire\} and Law, \{David J.\} and Larry Deaven and Bowcock, \{Anne M.\} and Collins, \{Francis S.\} and Weber, \{Barbara L.\} and Chandrasekharappa, \{Settara C.\}",

note = "Funding Information: The authors express their gratitude to the University of Michigan Human Genome Center for the use of many facilities during this study. This work was supported by NIH RO1 Grants CA-57601 and CA-61231 (B.W.), CA-60650 (A.B.), and CA-27632 (M.-C.K.). Piri Welcsh is supported in part by a Susan G. Komen Fellowship.",

year = "1995",

month = jan,

day = "1",

doi = "10.1016/0888-7543(95)80134-8",

language = "English",

volume = "25",

pages = "264--273",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

Couch, FJ, Castilla, LH, Xu, J, Abel, KJ, Welcsh, P, King, SE, Wong, L, Ho, PP, Merajver, S, Brody, LC, Yin, G, Hayes, ST, Gieser, LM, Flejter, WL, Glover, TW, Friedman, LS, Lynch, ED, Meza, JE, King, MC, Law, DJ, Deaven, L, Bowcock, AM, Collins, FS, Weber, BL & Chandrasekharappa, SC 1995, 'A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21', Genomics, vol. 25, no. 1, pp. 264-273. https://doi.org/10.1016/0888-7543(95)80134-8

TY - JOUR

T1 - A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21

AU - Couch, Fergus J.

AU - Castilla, Lucio H.

AU - Xu, Junzhe

AU - Abel, Kenneth J.

AU - Welcsh, Piri

AU - King, Stephanie E.

AU - Wong, Linghua

AU - Ho, Peggy P.

AU - Merajver, Sofia

AU - Brody, Lawrence C.

AU - Yin, Guiying

AU - Hayes, Steve T.

AU - Gieser, Linn M.

AU - Flejter, Wendy L.

AU - Glover, Thomas W.

AU - Friedman, Lori S.

AU - Lynch, Eric D.

AU - Meza, Jose E.

AU - King, Mary Claire

AU - Law, David J.

AU - Deaven, Larry

AU - Bowcock, Anne M.

AU - Collins, Francis S.

AU - Weber, Barbara L.

AU - Chandrasekharappa, Settara C.

N1 - Funding Information: The authors express their gratitude to the University of Michigan Human Genome Center for the use of many facilities during this study. This work was supported by NIH RO1 Grants CA-57601 and CA-61231 (B.W.), CA-60650 (A.B.), and CA-27632 (M.-C.K.). Piri Welcsh is supported in part by a Susan G. Komen Fellowship.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the interval has also been converted to a cosmid contig. Twenty-three PCR-based sequence-tagged sites (STSs) were mapped to these contigs, thereby confirming the order and overlap of individual clones. This complex physical map of the BRCA1 region was used to isolate genes by a number of gene identification techniques and to generate transcript maps of the region, as presented in the three accompanying manuscripts of Brody et al. (1995), Osborne-Lawrence et al. (1995), and Friedman et al. (1995).

AB - A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the interval has also been converted to a cosmid contig. Twenty-three PCR-based sequence-tagged sites (STSs) were mapped to these contigs, thereby confirming the order and overlap of individual clones. This complex physical map of the BRCA1 region was used to isolate genes by a number of gene identification techniques and to generate transcript maps of the region, as presented in the three accompanying manuscripts of Brody et al. (1995), Osborne-Lawrence et al. (1995), and Friedman et al. (1995).

UR - https://www.scopus.com/pages/publications/0028931908

U2 - 10.1016/0888-7543(95)80134-8

DO - 10.1016/0888-7543(95)80134-8

M3 - Article

C2 - 7774927

AN - SCOPUS:0028931908

SN - 0888-7543

VL - 25

SP - 264

EP - 273

JO - Genomics

JF - Genomics

IS - 1

ER -

A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21

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