A unique glycosphingolipid-splitting enzyme (ceramide-glycanase from leech) cleaves the linkage between the oligosaccharide and the ceramide

Su Chen Li, Rita Degasperi, James E. Muldrey, Yu Teh Li

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

A novel type of enzyme which hydrolyzes the linkage between the ceramide and the sugar chain in various glycosphingolipids has been found in the leech, Hirudo medicinalis. This enzyme releases the intact oligosaccharide from LacCer, GbOse3Cer, GbOse4Cer, GbOse5Cer, nLcOse4Cer, GM3, GM2, GM1, GD1a and GT1 with the concurrent release of ceramides. By using tritium-labeled GM1 as substrate we found the optimum pH of this enzyme to be between pH 4 and 5. Since the enzyme cleaves the linkage between the ceramide and the sugar chain in various glycosphingolipids with no apparent preference toward the sugar chain, we propose to call this enzyme ceramide-glycanase.

Original languageEnglish
Pages (from-to)346-352
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume141
Issue number1
DOIs
StatePublished - 26 Nov 1986
Externally publishedYes

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