TY - JOUR
T1 - A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
AU - Wei, Yuanchen
AU - Chen, Feng
AU - Zhang, Tao
AU - Chen, Deyong
AU - Jia, Xin
AU - Wang, Junbo
AU - Guo, Wei
AU - Chen, Jian
N1 - Funding Information:
The authors would like to acknowledge financial support from National Basic Research Program of China (973 Program, Grant No. 2014CB744602), Special Financial Grant from the China Postdoctoral Science Foundation (Grant No. 2013T60950), National Natural Science Foundation of China (Grant No. 81261120561, 61431019, 61201077, 81370420), National High Technology Research and Development Program of China (863 Program, Grant No. 2014AA093408) and Beijing NOVA Program.
PY - 2015/9/14
Y1 - 2015/9/14
N2 - This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments.
AB - This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments.
UR - http://www.scopus.com/inward/record.url?scp=84941585475&partnerID=8YFLogxK
U2 - 10.1038/srep14049
DO - 10.1038/srep14049
M3 - Article
C2 - 26365412
AN - SCOPUS:84941585475
SN - 2045-2322
VL - 5
JO - Scientific Reports
JF - Scientific Reports
M1 - 14049
ER -