@article{9c0018b1e6e64490a2fb6af0807a2d4f,
title = "A Translational Profiling Approach for the Molecular Characterization of CNS Cell Types",
abstract = "The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. Using bacterial artificial chromosome (BAC) transgenic mice that express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology for affinity purification of polysomal mRNAs from genetically defined cell populations in the brain. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neurons display vastly different translational profiles and present examples of the physiological significance of such differences. This genetically targeted translating ribosome affinity purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.",
keywords = "CELLBIO, MOLNEURO",
author = "Myriam Heiman and Anne Schaefer and Shiaoching Gong and Peterson, {Jayms D.} and Michelle Day and Ramsey, {Keri E.} and Mayte Su{\'a}rez-Fari{\~n}as and Cordelia Schwarz and Stephan, {Dietrich A.} and Surmeier, {D. James} and Paul Greengard and Nathaniel Heintz",
note = "Funding Information: We thank Helen Shio for performing electron microscopy, Angus Nairn and Cordelia Stearns for striatal slice experimental data, and Joseph Doyle and Joseph Dougherty for bacTRAP data and analysis, as well as members of the P. Greengard, N. Heintz, GENSAT, G. Blobel, and R. Darnell laboratories and Maxwell Heiman for comments and assistance. This work was supported by the National Institute on Drug Abuse (NIDA) fellowship 5F32DA021487 to M.H., German Research Foundation fellowship SCHA 1482/1-1 to A.S., National Institutes of Health/National Center for Research Resources grant 5UL1RR024143 to M.S.-F., National Institute of Neurological Disorders and Stroke grant NS34696 to D.J.S., grants from the F.M. Kirby Foundation, the Picower Foundation, the Jerry and Emily Spiegel Family Foundation, the Simons Foundation, the Peter Jay Sharp Foundation, and the Michael Stern Foundation, National Institute of Mental Health (NIMH) Conte Center grant MH074866 and NIDA grant DA10044 to P.G., the Simons Foundation, the Howard Hughes Medical Institute, the Adelson Medical Research Foundation, National Institute on Aging grant AG09464, and NIMH Conte Center grant MH074866 to N.H. ",
year = "2008",
month = nov,
day = "14",
doi = "10.1016/j.cell.2008.10.028",
language = "English",
volume = "135",
pages = "738--748",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}