TY - JOUR

T1 - A theoretical analysis of water transport through chondrocytes

AU - Ateshian, G. A.

AU - Costa, K. D.

AU - Hung, C. T.

N1 - Funding Information:
Acknowledgements This study was supported by funds from the National Institutes of Health (AR46532, EB004532).

PY - 2007/1

Y1 - 2007/1

N2 - Because of the avascular nature of adult cartilage, nutrients and waste products are transported to and from the chondrocytes by diffusion and convection through the extracellular matrix. The convective interstitial fluid flow within and around chondrocytes is poorly understood. This theoretical study demonstrates that the incorporation of a semi-permeable membrane when modeling the chondrocyte leads to the following findings: under mechanical loading of an isolated chondrocyte the intracellular fluid pressure is on the order of tens of Pascals and the transmembrane fluid outflow, on the order of picometers per second, takes several days to subside; consequently, the chondrocyte behaves practically as an incompressible solid whenever the loading duration is on the order of minutes or hours. When embedded in its extracellular matrix (ECM), the chondrocyte response is substantially different. Mechanical loading of the tissue leads to a fluid pressure difference between intracellular and extracellular compartments on the order of tens of kilopascals and the transmembrane outflow, on the order of a nanometer per second, subsides in about 1 h. The volume of the chondrocyte decreases concomitantly with that of the ECM. The interstitial fluid flow in the extracellular matrix is directed around the cell, with peak values on the order of tens of nanometers per second. The viscous fluid shear stress acting on the cell surface is several orders of magnitude smaller than the solid matrix shear stresses resulting from the ECM deformation. These results provide new insight toward our understanding of water transport in chondrocytes.

AB - Because of the avascular nature of adult cartilage, nutrients and waste products are transported to and from the chondrocytes by diffusion and convection through the extracellular matrix. The convective interstitial fluid flow within and around chondrocytes is poorly understood. This theoretical study demonstrates that the incorporation of a semi-permeable membrane when modeling the chondrocyte leads to the following findings: under mechanical loading of an isolated chondrocyte the intracellular fluid pressure is on the order of tens of Pascals and the transmembrane fluid outflow, on the order of picometers per second, takes several days to subside; consequently, the chondrocyte behaves practically as an incompressible solid whenever the loading duration is on the order of minutes or hours. When embedded in its extracellular matrix (ECM), the chondrocyte response is substantially different. Mechanical loading of the tissue leads to a fluid pressure difference between intracellular and extracellular compartments on the order of tens of kilopascals and the transmembrane outflow, on the order of a nanometer per second, subsides in about 1 h. The volume of the chondrocyte decreases concomitantly with that of the ECM. The interstitial fluid flow in the extracellular matrix is directed around the cell, with peak values on the order of tens of nanometers per second. The viscous fluid shear stress acting on the cell surface is several orders of magnitude smaller than the solid matrix shear stresses resulting from the ECM deformation. These results provide new insight toward our understanding of water transport in chondrocytes.

UR - http://www.scopus.com/inward/record.url?scp=33845436722&partnerID=8YFLogxK

U2 - 10.1007/s10237-006-0039-9

DO - 10.1007/s10237-006-0039-9

M3 - Article

C2 - 16705444

AN - SCOPUS:33845436722

VL - 6

SP - 91

EP - 101

JO - Biomechanics and Modeling in Mechanobiology

JF - Biomechanics and Modeling in Mechanobiology

SN - 1617-7959

IS - 1-2

ER -