10 Scopus citations


Determining the antigen specificities of the endogenous T-cell repertoire is important for screening naturally occurring or therapy-induced T-cell immunity and may help identify novel targets for T-cell-based therapies. Here, we describe a rapid, sensitive, and high-throughput protocol for expanding antigen-specific T cells from human peripheral blood mononuclear cells in vitro following peptide stimulation and detecting antigen-specific effector cytokine formation by flow cytometry. Our approach can be applied to examining specific T-cell subsets from various tissues. For complete details on the use and execution of this protocol, please refer to Roudko et al. (2020) and Cimen Bozkus et al. (2019).

Original languageEnglish
Article number100758
JournalSTAR Protocols
Issue number3
StatePublished - 17 Sep 2021


  • Cell culture
  • Cell-based Assays
  • Flow Cytometry/Mass Cytometry
  • High Throughput Screening
  • Immunology


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