A sudden increase in plasma epinephrine levels transiently enhances platelet deposition on severely damaged arterial wall. Studies in a porcine model

Lina Badimon, José Martínez-González, Teresita Royo, Riitta Lassila, José Juan Badimon

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37 Scopus citations

Abstract

Epidemiologic evidence has shown that sympathoadrenal activation plays a triggering role in the onset of acute coronary syndromes. However, its mechanism is not yet clearly understood. The aim of this study was to assess the effect of a sudden increase in epinephrine on platelet deposition on severely damaged vessel wall at shear rate conditions modelling stenotic vessels in the porcine model. The selected epinephrine concentrations (0.5 μmol/l-1 mmol/1) alone or in combination with collagen or ADP did not affect platelet aggregation in vitro either in whole blood or in PRP, although porcine platelets express α2-adrenergic receptors as assessed by PCR. In vitro and ex vivo perfusion experiments were performed using the Badimon chamber at high shear rate conditions (1690 s-1). In vitro, epinephrine (130 nmol/1) increased platelet deposition on severely damaged vessel wall (exposing tunica media; ≃ 1.6-fold, p < 0.05) or immobilized collagen (2.2-fold, p < 0.01). Ex vivo perfusion experiments were performed from animals that received intravenous epinephrine infusion for one hour at a low (0.3 μg/kg/min; ≃ 17 nmol/l in plasma, at 20 min of the infusion) and a high dose (1.0 μg/kg/min; ≃ 106 nmol/1 in plasma, at 20 min of the infusion). Only the low dose temporarily increased platelet deposition on severely damaged vessel wall during the first 30 min of infusion [2.4-fold (p < 0.05) and 4.2-fold (p < 0.01) at 10 and 30 min of the infusion respectively] declining afterwards. Thus, in flow conditions typical of atherosclerotic arteries, a sudden physiological release of epinephrine can temporarily enhance platelet deposition on severely damaged vessel wall while an extensive exposure leads to refractoriness.

Original languageEnglish
Pages (from-to)1736-1742
Number of pages7
JournalThrombosis and Haemostasis
Volume82
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Epinephrine
  • Pig
  • Platelets
  • Vascular damage
  • Vessel wall

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