A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells

Kristinn P. Magnusson; Janos Minarovits; George Klein; Klas G. Wiman

doi:10.1016/0378-1119(95)00338-7

A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells

Kristinn P. Magnusson
, Janos Minarovits
, George Klein
, Klas G. Wiman

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3′ in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg²⁵⁸→Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.

Original language	English
Pages (from-to)	231-234
Number of pages	4
Journal	Gene
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00338-7
State	Published - 11 Sep 1995
Externally published	Yes

Keywords

AMV
Alternative splicing
P53
PCR
S-ADH
SEWA-AS12-ADH mouse sarcoma cell line
aa
amino acids (s)
avian myeloblastosis virus
base pair(s)
bp
gene encoding P53
insertion
mAb
monoclonal antibody(ies)
murinep53
nt
nucleotide(s)
p53
polymerase chain reaction
tumor suppressor
tumor suppressors
tumorigenesis
wild type
wt

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10.1016/0378-1119(95)00338-7

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title = "A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells",

abstract = "The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3′ in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg258→Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.",

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author = "Magnusson, \{Kristinn P.\} and Janos Minarovits and George Klein and Wiman, \{Klas G.\}",

year = "1995",

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doi = "10.1016/0378-1119(95)00338-7",

language = "English",

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pages = "231--234",

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T1 - A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells

AU - Magnusson, Kristinn P.

AU - Minarovits, Janos

AU - Klein, George

AU - Wiman, Klas G.

PY - 1995/9/11

Y1 - 1995/9/11

N2 - The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3′ in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg258→Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.

AB - The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3′ in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg258→Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.

KW - AMV

KW - Alternative splicing

KW - P53

KW - PCR

KW - S-ADH

KW - SEWA-AS12-ADH mouse sarcoma cell line

KW - aa

KW - amino acids (s)

KW - avian myeloblastosis virus

KW - base pair(s)

KW - bp

KW - gene encoding P53

KW - insertion

KW - mAb

KW - monoclonal antibody(ies)

KW - murinep53

KW - nt

KW - nucleotide(s)

KW - p53

KW - polymerase chain reaction

KW - tumor suppressor

KW - tumor suppressors

KW - tumorigenesis

KW - wild type

KW - wt

UR - https://www.scopus.com/pages/publications/0029149817

U2 - 10.1016/0378-1119(95)00338-7

DO - 10.1016/0378-1119(95)00338-7

M3 - Article

C2 - 7557434

AN - SCOPUS:0029149817

SN - 0378-1119

VL - 162

SP - 231

EP - 234

JO - Gene

JF - Gene

IS - 2

ER -

A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells

Abstract

Keywords

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