A snapshot of ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination

Jordan Kovacev; Kenneth Wu; Donald E. Spratt; Robert A. Chong; Chan Lee; Jaladhi Nayak; Gary S. Shaw; Zhen Qiang Pan

doi:10.1074/jbc.M113.530576

A snapshot of ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination

Jordan Kovacev, Kenneth Wu, Donald E. Spratt, Robert A. Chong, Chan Lee, Jaladhi Nayak, Gary S. Shaw, Zhen Qiang Pan

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background: Polyubiquitin chains are signaling polypeptides altering the fate of substrates. Results: A Lys-48-ubiquitin chain of a length greater than four, but not its Lys-63 linkage counterparts, slowed the rate of additional ubiquitin conjugation. Conclusion: The ubiquitin chain length and linkage may impact kinetic rates of chain elongation. Significance: Our findings suggest a self-restraining mechanism that limits Lys-48-polyubiquitination.

Original language	English
Pages (from-to)	7068-7081
Number of pages	14
Journal	Journal of Biological Chemistry
Volume	289
Issue number	10
DOIs	https://doi.org/10.1074/jbc.M113.530576
State	Published - 7 Mar 2014

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abstract = "Background: Polyubiquitin chains are signaling polypeptides altering the fate of substrates. Results: A Lys-48-ubiquitin chain of a length greater than four, but not its Lys-63 linkage counterparts, slowed the rate of additional ubiquitin conjugation. Conclusion: The ubiquitin chain length and linkage may impact kinetic rates of chain elongation. Significance: Our findings suggest a self-restraining mechanism that limits Lys-48-polyubiquitination.",

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T2 - Lysine 48-tetra-ubiquitin slows down ubiquitination

AU - Kovacev, Jordan

AU - Wu, Kenneth

AU - Spratt, Donald E.

AU - Chong, Robert A.

AU - Lee, Chan

AU - Nayak, Jaladhi

AU - Shaw, Gary S.

AU - Pan, Zhen Qiang

PY - 2014/3/7

Y1 - 2014/3/7

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AB - Background: Polyubiquitin chains are signaling polypeptides altering the fate of substrates. Results: A Lys-48-ubiquitin chain of a length greater than four, but not its Lys-63 linkage counterparts, slowed the rate of additional ubiquitin conjugation. Conclusion: The ubiquitin chain length and linkage may impact kinetic rates of chain elongation. Significance: Our findings suggest a self-restraining mechanism that limits Lys-48-polyubiquitination.

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A snapshot of ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination

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