Abstract
Background: Polyubiquitin chains are signaling polypeptides altering the fate of substrates. Results: A Lys-48-ubiquitin chain of a length greater than four, but not its Lys-63 linkage counterparts, slowed the rate of additional ubiquitin conjugation. Conclusion: The ubiquitin chain length and linkage may impact kinetic rates of chain elongation. Significance: Our findings suggest a self-restraining mechanism that limits Lys-48-polyubiquitination.
Original language | English |
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Pages (from-to) | 7068-7081 |
Number of pages | 14 |
Journal | Journal of Biological Chemistry |
Volume | 289 |
Issue number | 10 |
DOIs | |
State | Published - 7 Mar 2014 |