A snapshot of ubiquitin chain elongation: Lysine 48-tetra-ubiquitin slows down ubiquitination

Jordan Kovacev, Kenneth Wu, Donald E. Spratt, Robert A. Chong, Chan Lee, Jaladhi Nayak, Gary S. Shaw, Zhen Qiang Pan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: Polyubiquitin chains are signaling polypeptides altering the fate of substrates. Results: A Lys-48-ubiquitin chain of a length greater than four, but not its Lys-63 linkage counterparts, slowed the rate of additional ubiquitin conjugation. Conclusion: The ubiquitin chain length and linkage may impact kinetic rates of chain elongation. Significance: Our findings suggest a self-restraining mechanism that limits Lys-48-polyubiquitination.

Original languageEnglish
Pages (from-to)7068-7081
Number of pages14
JournalJournal of Biological Chemistry
Volume289
Issue number10
DOIs
StatePublished - 7 Mar 2014

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