A single p34(cdc2) protein kinase (encoded by nim X(cdc2))is required at G1 and G2 in Aspergillus nidulans

A. H. Osmani, N. Van Peij, M. Mischke, M. J. O'Connell, S. A. Osmani

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68 Scopus citations

Abstract

We have cloned and sequenced a homolog of cdc2 from Aspergillus nidulans that can complement the Schizosaccharomyces pombe cdc2-33 mutation. The gene was deleted and is required for continued nuclear DNA replication but not for mitochondrial DNA replication. Three different temperature-sensitive alleles were generated by reverse genetics. All of the mutations generate the nim phenotype of A. nidulans. The new gene was designated nimX(cdc2) as it is not allelic to any of the other nim genes (nimA to nimW) of A. nidulans. Reciprocal shift experiments place an essential function for nimX(cdc2) in G1 and G2. Antipeptide antibodies were generated that detect NIMX(cdc2), and antisera were also generated to detect NIME(cyclinB). The two p34(cdc2) protein species previously detected in A. nidulans, p34 and p37, both precipitate using NIMX(cdc2) C-terminus-specific antibodies but only p34 co-precipitates with NIME(cyclinB). Dephosphorylation of denatured p34 converts it to the p37 form, showing p37 to be the non-phosphorylated form of NIMX(cdc2). The phosphorylation of p34 is therefore associated with its interaction with NIME(cyclinB).

Original languageEnglish
Pages (from-to)1519-1528
Number of pages10
JournalJournal of Cell Science
Volume107
Issue number6
StatePublished - 1994
Externally publishedYes

Keywords

  • Aspergillus nidulans
  • Cell cycle regulation
  • Cyclin B
  • nimE(cyclinB)
  • nimx(cdc2)
  • p34(cdc2)

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