A simple polymerase chain reaction apparatus based on a computer printer

Aurelian Radu

doi:10.1016/0165-022X(91)90002-E

A simple polymerase chain reaction apparatus based on a computer printer

Aurelian Radu

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a very simple laboratory-made polymerase chain reaction (PCR) apparatus. The reaction tubes are placed in a holder fixed through a mechanical arm to the tape cartridge of a computer printer. A computer controls the horizontal movement of the tube carrier by sending the proper printing commands. The holder is raised or lowered by a frame fixed to the paper-advancing roller of the printer. The system allows the programmed movement of the test tubes within the holder, successively through three thermal baths placed in front of the printer. DNA from single lambda gtll lysis plaques was successfully amplified with this system in our laboratory.

Original language	English
Pages (from-to)	275-282
Number of pages	8
Journal	Journal of Biochemical and Biophysical Methods
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/0165-022X(91)90002-E
State	Published - Dec 1991
Externally published	Yes

Keywords

Computer control
DNA amplification
Polymerase chain reaction

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10.1016/0165-022X(91)90002-E

Cite this

@article{227b21cea6ce4e368ac1338a62341899,

title = "A simple polymerase chain reaction apparatus based on a computer printer",

abstract = "We describe a very simple laboratory-made polymerase chain reaction (PCR) apparatus. The reaction tubes are placed in a holder fixed through a mechanical arm to the tape cartridge of a computer printer. A computer controls the horizontal movement of the tube carrier by sending the proper printing commands. The holder is raised or lowered by a frame fixed to the paper-advancing roller of the printer. The system allows the programmed movement of the test tubes within the holder, successively through three thermal baths placed in front of the printer. DNA from single lambda gtll lysis plaques was successfully amplified with this system in our laboratory.",

keywords = "Computer control, DNA amplification, Polymerase chain reaction",

author = "Aurelian Radu",

note = "Funding Information: The author wishes to thank to Prof. D. Kerjaschki and Dr S. Binder (University of Vienna) for generous support, D. Paul (construction assistance), M. Schean and C. Neacsu (graphic work), L. Barbulescu (typing and editing), and G. Stefan and V. Ionescu (photography). This work was supported by the Ministry of Education, Romania, and by the National Institutes of Health (U.S.A.) grant HL-26343.",

year = "1991",

month = dec,

doi = "10.1016/0165-022X(91)90002-E",

language = "English",

volume = "23",

pages = "275--282",

journal = "Journal of Biochemical and Biophysical Methods",

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publisher = "Elsevier B.V.",

number = "4",

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T1 - A simple polymerase chain reaction apparatus based on a computer printer

AU - Radu, Aurelian

N1 - Funding Information: The author wishes to thank to Prof. D. Kerjaschki and Dr S. Binder (University of Vienna) for generous support, D. Paul (construction assistance), M. Schean and C. Neacsu (graphic work), L. Barbulescu (typing and editing), and G. Stefan and V. Ionescu (photography). This work was supported by the Ministry of Education, Romania, and by the National Institutes of Health (U.S.A.) grant HL-26343.

PY - 1991/12

Y1 - 1991/12

N2 - We describe a very simple laboratory-made polymerase chain reaction (PCR) apparatus. The reaction tubes are placed in a holder fixed through a mechanical arm to the tape cartridge of a computer printer. A computer controls the horizontal movement of the tube carrier by sending the proper printing commands. The holder is raised or lowered by a frame fixed to the paper-advancing roller of the printer. The system allows the programmed movement of the test tubes within the holder, successively through three thermal baths placed in front of the printer. DNA from single lambda gtll lysis plaques was successfully amplified with this system in our laboratory.

AB - We describe a very simple laboratory-made polymerase chain reaction (PCR) apparatus. The reaction tubes are placed in a holder fixed through a mechanical arm to the tape cartridge of a computer printer. A computer controls the horizontal movement of the tube carrier by sending the proper printing commands. The holder is raised or lowered by a frame fixed to the paper-advancing roller of the printer. The system allows the programmed movement of the test tubes within the holder, successively through three thermal baths placed in front of the printer. DNA from single lambda gtll lysis plaques was successfully amplified with this system in our laboratory.

KW - Computer control

KW - DNA amplification

KW - Polymerase chain reaction

UR - https://www.scopus.com/pages/publications/0025952220

U2 - 10.1016/0165-022X(91)90002-E

DO - 10.1016/0165-022X(91)90002-E

M3 - Article

C2 - 1770198

AN - SCOPUS:0025952220

SN - 0165-022X

VL - 23

SP - 275

EP - 282

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 4

ER -

A simple polymerase chain reaction apparatus based on a computer printer

Abstract

Keywords

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