A serological assay to detect SARS-CoV-2 seroconversion in humans

Fatima Amanat; Daniel Stadlbauer; Shirin Strohmeier; Thi H.O. Nguyen; Veronika Chromikova; Meagan McMahon; Kaijun Jiang; Guha Asthagiri Arunkumar; Denise Jurczyszak; Jose Polanco; Maria Bermudez-Gonzalez; Giulio Kleiner; Teresa Aydillo; Lisa Miorin; Daniel S. Fierer; Luz Amarilis Lugo; Erna Milunka Kojic; Jonathan Stoever; Sean T.H. Liu; Charlotte Cunningham-Rundles; Philip L. Felgner; Thomas Moran; Adolfo García-Sastre; Daniel Caplivski; Allen C. Cheng; Katherine Kedzierska; Olli Vapalahti; Jussi M. Hepojoki; Viviana Simon; Florian Krammer

doi:10.1038/s41591-020-0913-5

A serological assay to detect SARS-CoV-2 seroconversion in humans

Fatima Amanat, Daniel Stadlbauer, Shirin Strohmeier, Thi H.O. Nguyen, Veronika Chromikova, Meagan McMahon, Kaijun Jiang, Guha Asthagiri Arunkumar, Denise Jurczyszak, Jose Polanco, Maria Bermudez-Gonzalez, Giulio Kleiner, Teresa Aydillo, Lisa Miorin, Daniel S. Fierer, Luz Amarilis Lugo, Erna Milunka Kojic, Jonathan Stoever, Sean T.H. Liu, Charlotte Cunningham-RundlesPhilip L. Felgner, Thomas Moran, Adolfo García-Sastre, Daniel Caplivski, Allen C. Cheng, Katherine Kedzierska, Olli Vapalahti, Jussi M. Hepojoki, Viviana Simon, Florian Krammer

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Abstract

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

Original language	English
Pages (from-to)	1033-1036
Number of pages	4
Journal	Nature Medicine
Volume	26
Issue number	7
DOIs	https://doi.org/10.1038/s41591-020-0913-5
State	Published - 1 Jul 2020

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Amanat, F., Stadlbauer, D., Strohmeier, S., Nguyen, T. H. O., Chromikova, V., McMahon, M., Jiang, K., Arunkumar, G. A., Jurczyszak, D., Polanco, J., Bermudez-Gonzalez, M., Kleiner, G., Aydillo, T., Miorin, L., Fierer, D. S., Lugo, L. A., Kojic, E. M., Stoever, J., Liu, S. T. H., ... Krammer, F. (2020). A serological assay to detect SARS-CoV-2 seroconversion in humans. Nature Medicine, 26(7), 1033-1036. https://doi.org/10.1038/s41591-020-0913-5

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title = "A serological assay to detect SARS-CoV-2 seroconversion in humans",

abstract = "Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.",

author = "Fatima Amanat and Daniel Stadlbauer and Shirin Strohmeier and Nguyen, {Thi H.O.} and Veronika Chromikova and Meagan McMahon and Kaijun Jiang and Arunkumar, {Guha Asthagiri} and Denise Jurczyszak and Jose Polanco and Maria Bermudez-Gonzalez and Giulio Kleiner and Teresa Aydillo and Lisa Miorin and Fierer, {Daniel S.} and Lugo, {Luz Amarilis} and Kojic, {Erna Milunka} and Jonathan Stoever and Liu, {Sean T.H.} and Charlotte Cunningham-Rundles and Felgner, {Philip L.} and Thomas Moran and Adolfo Garc{\'i}a-Sastre and Daniel Caplivski and Cheng, {Allen C.} and Katherine Kedzierska and Olli Vapalahti and Hepojoki, {Jussi M.} and Viviana Simon and Florian Krammer",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2020",

month = jul,

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doi = "10.1038/s41591-020-0913-5",

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Amanat, F, Stadlbauer, D, Strohmeier, S, Nguyen, THO, Chromikova, V, McMahon, M, Jiang, K, Arunkumar, GA, Jurczyszak, D, Polanco, J, Bermudez-Gonzalez, M, Kleiner, G, Aydillo, T , Miorin, L , Fierer, DS, Lugo, LA, Kojic, EM, Stoever, J, Liu, STH , Cunningham-Rundles, C, Felgner, PL, Moran, T, García-Sastre, A , Caplivski, D, Cheng, AC, Kedzierska, K, Vapalahti, O, Hepojoki, JM, Simon, V & Krammer, F 2020, 'A serological assay to detect SARS-CoV-2 seroconversion in humans', Nature Medicine, vol. 26, no. 7, pp. 1033-1036. https://doi.org/10.1038/s41591-020-0913-5

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T1 - A serological assay to detect SARS-CoV-2 seroconversion in humans

AU - Amanat, Fatima

AU - Stadlbauer, Daniel

AU - Strohmeier, Shirin

AU - Nguyen, Thi H.O.

AU - Chromikova, Veronika

AU - McMahon, Meagan

AU - Jiang, Kaijun

AU - Arunkumar, Guha Asthagiri

AU - Jurczyszak, Denise

AU - Polanco, Jose

AU - Bermudez-Gonzalez, Maria

AU - Kleiner, Giulio

AU - Aydillo, Teresa

AU - Miorin, Lisa

AU - Fierer, Daniel S.

AU - Lugo, Luz Amarilis

AU - Kojic, Erna Milunka

AU - Stoever, Jonathan

AU - Liu, Sean T.H.

AU - Cunningham-Rundles, Charlotte

AU - Felgner, Philip L.

AU - Moran, Thomas

AU - García-Sastre, Adolfo

AU - Caplivski, Daniel

AU - Cheng, Allen C.

AU - Kedzierska, Katherine

AU - Vapalahti, Olli

AU - Hepojoki, Jussi M.

AU - Simon, Viviana

AU - Krammer, Florian

PY - 2020/7/1

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N2 - Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

AB - Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

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SP - 1033

EP - 1036

JO - Nature Medicine

JF - Nature Medicine

IS - 7

ER -

A serological assay to detect SARS-CoV-2 seroconversion in humans

Abstract

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