TY - JOUR
T1 - A serological assay to detect SARS-CoV-2 seroconversion in humans
AU - Amanat, Fatima
AU - Stadlbauer, Daniel
AU - Strohmeier, Shirin
AU - Nguyen, Thi H.O.
AU - Chromikova, Veronika
AU - McMahon, Meagan
AU - Jiang, Kaijun
AU - Arunkumar, Guha Asthagiri
AU - Jurczyszak, Denise
AU - Polanco, Jose
AU - Bermudez-Gonzalez, Maria
AU - Kleiner, Giulio
AU - Aydillo, Teresa
AU - Miorin, Lisa
AU - Fierer, Daniel S.
AU - Lugo, Luz Amarilis
AU - Kojic, Erna Milunka
AU - Stoever, Jonathan
AU - Liu, Sean T.H.
AU - Cunningham-Rundles, Charlotte
AU - Felgner, Philip L.
AU - Moran, Thomas
AU - García-Sastre, Adolfo
AU - Caplivski, Daniel
AU - Cheng, Allen C.
AU - Kedzierska, Katherine
AU - Vapalahti, Olli
AU - Hepojoki, Jussi M.
AU - Simon, Viviana
AU - Krammer, Florian
N1 - Funding Information:
We thank Y.-Z. Zhang (Fudan University) and E. Holmes (University of Sydney) for sharing the sequence of the first SARS-CoV-2 isolate in a very timely manner. We thank J. Garlick and J. Roney (Alfred Hospital, Melbourne) for data and specimen collection, N. Aboelregal for making many different NHIG products (Mount Sinai) available and L. Martinez-Sobrido (Texas Biomedical Research Institute) for initially characterizing mAb 1C7. We are also thankful to Genewiz for speeding up gene synthesis for this project, and for being very accommodating to our needs. Furthermore, we thank D. Tidmore for help with ordering primers quickly, and finally S. Dong for commuting to New Jersey on several occasions to pick up reagents from Genewiz. We also thank the Mount Sinai Health System Translational Science Hub ‘ConduITS’ (NIH grant U54TR001433) for supporting sample collection. The work of the Personalized Virology Initiative is supported by institutional funds and philanthropic donations. This work was partially supported by the NIAID Centers of Excellence for Influenza Research and Surveillance (CEIRS; contract HHSN272201400008C to F.K. and A.G.-S.), the Collaborative Influenza Vaccine Innovation Centers (CIVIC; contract 75N93019C00051 to F.K. and A.G.-S.), Open Philanthropy, the Australian National Health and Medical Research Council (NHMRC Program Grant 1071916 and NHMRC Research Fellowship Level B (1102792) to K.K.), the Academy of Finland to O.V. and J.M.H., as well as Jane and Aatos Erkko Foundation and Helsinki University Hospital Funds to O.V. Furthermore, we thank our generous community for providing essential funds and support for our SARS-CoV-2 and COVID-19 research efforts. The following reagent was deposited by the Centers for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, NR-52281. Finally, we thank all of the study participants for their contribution to the research. We wish the patients with COVID-19 a speedy recovery.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.
AB - Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.
UR - http://www.scopus.com/inward/record.url?scp=85084429680&partnerID=8YFLogxK
U2 - 10.1038/s41591-020-0913-5
DO - 10.1038/s41591-020-0913-5
M3 - Article
C2 - 32398876
AN - SCOPUS:85084429680
VL - 26
SP - 1033
EP - 1036
JO - Nature Medicine
JF - Nature Medicine
SN - 1078-8956
IS - 7
ER -