A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

Xi Dai; Tao Zhang; Jun Ying Miao; Bao Xiang Zhao

doi:10.1016/j.snb.2015.09.106

A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

Xi Dai, Tao Zhang, Jun Ying Miao, Bao Xiang Zhao

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10^-8 M and two-photon 10^-7 M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.

Original language	English
Pages (from-to)	274-279
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	223
DOIs	https://doi.org/10.1016/j.snb.2015.09.106
State	Published - 1 Feb 2016
Externally published	Yes

Keywords

Aqueous solution
Cell imaging
Ratiometric fluorescence
Two-photon

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10.1016/j.snb.2015.09.106

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title = "A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution",

abstract = "A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10-8 M and two-photon 10-7 M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.",

keywords = "Aqueous solution, Cell imaging, Ratiometric fluorescence, Two-photon",

author = "Xi Dai and Tao Zhang and Miao, {Jun Ying} and Zhao, {Bao Xiang}",

year = "2016",

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T1 - A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

AU - Dai, Xi

AU - Zhang, Tao

AU - Miao, Jun Ying

AU - Zhao, Bao Xiang

PY - 2016/2/1

Y1 - 2016/2/1

N2 - A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10-8 M and two-photon 10-7 M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.

AB - A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10-8 M and two-photon 10-7 M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.

KW - Aqueous solution

KW - Cell imaging

KW - Ratiometric fluorescence

KW - Two-photon

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SP - 274

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

Abstract

Keywords

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