A rapid method for quantitating the frequency of peripheral blood cells containing hiv-1 dna

Mark C. Poznansky; Bruce Walker; William A. Haseltine; Joseph Sodroski; Erik Langhoff

A rapid method for quantitating the frequency of peripheral blood cells containing hiv-1 dna

Mark C. Poznansky, Bruce Walker, William A. Haseltine, Joseph Sodroski, Erik Langhoff

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

This article describes a novel approach to quantitative polymerase chain reactions (PCR). The technique is simple to execute, can be performed in a single tube, and is suitable for automation. In addition, the counting and lysis of low numbers of cells (1-100) can be confirmed by phase contrast microscopy. In this study, the technique was used to determine the frequency of occurrence of DNA from the human immunodeficiency virus (type 1) in leukocyte subsets of peripheral blood mononuclear cells (PBMC) from seropositive individuals. It was confirmed that the frequency of infected CD4⁺ T cells varied from 0.01 to 0.0001. In addition, HIV-1 DNA was detected in the B cell/dendritic cell-enriched subpopulation in four of nine HIV-1-positive individuals in the study.

Original language	English
Pages (from-to)	368-373
Number of pages	6
Journal	Journal of Acquired Immune Deficiency Syndromes
Volume	4
Issue number	4
State	Published - Apr 1991
Externally published	Yes

Keywords

CD4+ T cells
HIV-1 DNA
Human immunodeficiency virus type 1
Peripheral blood mononuclear cells
Polymerase chain reaction

Cite this

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title = "A rapid method for quantitating the frequency of peripheral blood cells containing hiv-1 dna",

abstract = "This article describes a novel approach to quantitative polymerase chain reactions (PCR). The technique is simple to execute, can be performed in a single tube, and is suitable for automation. In addition, the counting and lysis of low numbers of cells (1-100) can be confirmed by phase contrast microscopy. In this study, the technique was used to determine the frequency of occurrence of DNA from the human immunodeficiency virus (type 1) in leukocyte subsets of peripheral blood mononuclear cells (PBMC) from seropositive individuals. It was confirmed that the frequency of infected CD4+ T cells varied from 0.01 to 0.0001. In addition, HIV-1 DNA was detected in the B cell/dendritic cell-enriched subpopulation in four of nine HIV-1-positive individuals in the study.",

keywords = "CD4+ T cells, HIV-1 DNA, Human immunodeficiency virus type 1, Peripheral blood mononuclear cells, Polymerase chain reaction",

author = "Poznansky, \{Mark C.\} and Bruce Walker and Haseltine, \{William A.\} and Joseph Sodroski and Erik Langhoff",

year = "1991",

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T1 - A rapid method for quantitating the frequency of peripheral blood cells containing hiv-1 dna

AU - Poznansky, Mark C.

AU - Walker, Bruce

AU - Haseltine, William A.

AU - Sodroski, Joseph

AU - Langhoff, Erik

PY - 1991/4

Y1 - 1991/4

N2 - This article describes a novel approach to quantitative polymerase chain reactions (PCR). The technique is simple to execute, can be performed in a single tube, and is suitable for automation. In addition, the counting and lysis of low numbers of cells (1-100) can be confirmed by phase contrast microscopy. In this study, the technique was used to determine the frequency of occurrence of DNA from the human immunodeficiency virus (type 1) in leukocyte subsets of peripheral blood mononuclear cells (PBMC) from seropositive individuals. It was confirmed that the frequency of infected CD4+ T cells varied from 0.01 to 0.0001. In addition, HIV-1 DNA was detected in the B cell/dendritic cell-enriched subpopulation in four of nine HIV-1-positive individuals in the study.

AB - This article describes a novel approach to quantitative polymerase chain reactions (PCR). The technique is simple to execute, can be performed in a single tube, and is suitable for automation. In addition, the counting and lysis of low numbers of cells (1-100) can be confirmed by phase contrast microscopy. In this study, the technique was used to determine the frequency of occurrence of DNA from the human immunodeficiency virus (type 1) in leukocyte subsets of peripheral blood mononuclear cells (PBMC) from seropositive individuals. It was confirmed that the frequency of infected CD4+ T cells varied from 0.01 to 0.0001. In addition, HIV-1 DNA was detected in the B cell/dendritic cell-enriched subpopulation in four of nine HIV-1-positive individuals in the study.

KW - CD4+ T cells

KW - HIV-1 DNA

KW - Human immunodeficiency virus type 1

KW - Peripheral blood mononuclear cells

KW - Polymerase chain reaction

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M3 - Article

C2 - 1672549

AN - SCOPUS:0026090566

SN - 1525-4135

VL - 4

SP - 368

EP - 373

JO - Journal of Acquired Immune Deficiency Syndromes

JF - Journal of Acquired Immune Deficiency Syndromes

IS - 4

ER -

A rapid method for quantitating the frequency of peripheral blood cells containing hiv-1 dna

Abstract

Keywords

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