A Rapid Automated Stathmokinetic Method For Determination of In Vitro Cell Cycle Transit Times

G. M. Dosik; B. Barlogie; R. Allen White; W. Göhde; B. Drewinko

doi:10.1111/j.1365-2184.1981.tb00517.x

A Rapid Automated Stathmokinetic Method For Determination of In Vitro Cell Cycle Transit Times

G. M. Dosik, B. Barlogie, R. Allen White, W. Göhde, B. Drewinko

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

To provide a rapid method for examining cell cycle dynamics, we utilized continuous exposure of Chinese hamster ovary cells and human colon cancer cells to colcemid to block cycling cells in metaphase, suppressing re‐entry into G₁. Changes in cell cycle compartment distribution were monitored by DNA flow cytometry. Analysis of the rate of G₂+ M compartment accumulation after addition of colcemid permitted calculation of all cycle transit parameters. These compared favorably with data in the same cell lines determined by the fraction of labeled mitoses technique. Serial assessment of DNA flow cytometry after addition of colcemid permits rapid quantitation of cycle traverse rates.

Original language	English
Pages (from-to)	121-134
Number of pages	14
Journal	Cell Proliferation
Volume	14
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2184.1981.tb00517.x
State	Published - Mar 1981
Externally published	Yes

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title = "A Rapid Automated Stathmokinetic Method For Determination of In Vitro Cell Cycle Transit Times",

abstract = "To provide a rapid method for examining cell cycle dynamics, we utilized continuous exposure of Chinese hamster ovary cells and human colon cancer cells to colcemid to block cycling cells in metaphase, suppressing re‐entry into G1. Changes in cell cycle compartment distribution were monitored by DNA flow cytometry. Analysis of the rate of G2+ M compartment accumulation after addition of colcemid permitted calculation of all cycle transit parameters. These compared favorably with data in the same cell lines determined by the fraction of labeled mitoses technique. Serial assessment of DNA flow cytometry after addition of colcemid permits rapid quantitation of cycle traverse rates.",

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AU - Göhde, W.

AU - Drewinko, B.

PY - 1981/3

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N2 - To provide a rapid method for examining cell cycle dynamics, we utilized continuous exposure of Chinese hamster ovary cells and human colon cancer cells to colcemid to block cycling cells in metaphase, suppressing re‐entry into G1. Changes in cell cycle compartment distribution were monitored by DNA flow cytometry. Analysis of the rate of G2+ M compartment accumulation after addition of colcemid permitted calculation of all cycle transit parameters. These compared favorably with data in the same cell lines determined by the fraction of labeled mitoses technique. Serial assessment of DNA flow cytometry after addition of colcemid permits rapid quantitation of cycle traverse rates.

AB - To provide a rapid method for examining cell cycle dynamics, we utilized continuous exposure of Chinese hamster ovary cells and human colon cancer cells to colcemid to block cycling cells in metaphase, suppressing re‐entry into G1. Changes in cell cycle compartment distribution were monitored by DNA flow cytometry. Analysis of the rate of G2+ M compartment accumulation after addition of colcemid permitted calculation of all cycle transit parameters. These compared favorably with data in the same cell lines determined by the fraction of labeled mitoses technique. Serial assessment of DNA flow cytometry after addition of colcemid permits rapid quantitation of cycle traverse rates.

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A Rapid Automated Stathmokinetic Method For Determination of In Vitro Cell Cycle Transit Times

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