A rapid and accurate procedure for assaying DNA polymerase, RNA polymerase, or ribosome dependent protein synthesis

Bernard I. Weinstein, Nina Bhardwaj, Heng Chun Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A descending paper chromatographic procedure for separating trichloracetic acid soluble from precipitable material is described. Its use for assaying DNA, RNA, or protein synthesis from a variety of sources with various states of purity attests to its general applicability to reactions of interest to molecular biologists. Large numbers of analyses can be carried out in a short period of time without compromising accuracy or reliability. The use of this procedure for still more enzymes, those involved in modifying DNA, RNA, or proteins, is discussed.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalAnalytical Biochemistry
Volume68
Issue number1
DOIs
StatePublished - Sep 1975
Externally publishedYes

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