A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters

Graham C.R. Ellis-Davies

doi:10.1038/nprot.2010.193

A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters

Graham C.R. Ellis-Davies

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

This protocol describes a method for efficient chemical synthesis of dinitroindolinyl derivatives of glutamate and Î 3-aminobutyric acid. These caged neurotransmitters are currently the most chemically and photochemically efficient probes for two-photon photolysis in living brain slices. The protocol only requires basic organic synthesis equipment, and no silica gel column chromatography or NMR spectroscopy is needed at any stage. HPLC is used to purify the caged transmitters at the end of the syntheses. Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestly equipped chemistry laboratory.

Original language	English
Pages (from-to)	314-326
Number of pages	13
Journal	Nature Protocols
Volume	6
Issue number	3
DOIs	https://doi.org/10.1038/nprot.2010.193
State	Published - Mar 2011

Access to Document

10.1038/nprot.2010.193

Cite this

@article{defd18cd9cbb4d40b9340cd95ef24f49,

title = "A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters",

abstract = "This protocol describes a method for efficient chemical synthesis of dinitroindolinyl derivatives of glutamate and {\^I} 3-aminobutyric acid. These caged neurotransmitters are currently the most chemically and photochemically efficient probes for two-photon photolysis in living brain slices. The protocol only requires basic organic synthesis equipment, and no silica gel column chromatography or NMR spectroscopy is needed at any stage. HPLC is used to purify the caged transmitters at the end of the syntheses. Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestly equipped chemistry laboratory.",

author = "Ellis-Davies, {Graham C.R.}",

note = "Funding Information: acknowleDGMents This work was supported by a grant from the US National Institutes of Health (GM53395) to G.C.R.E.-D.",

year = "2011",

month = mar,

doi = "10.1038/nprot.2010.193",

language = "English",

volume = "6",

pages = "314--326",

journal = "Nature Protocols",

issn = "1754-2189",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters

AU - Ellis-Davies, Graham C.R.

N1 - Funding Information: acknowleDGMents This work was supported by a grant from the US National Institutes of Health (GM53395) to G.C.R.E.-D.

PY - 2011/3

Y1 - 2011/3

N2 - This protocol describes a method for efficient chemical synthesis of dinitroindolinyl derivatives of glutamate and Î 3-aminobutyric acid. These caged neurotransmitters are currently the most chemically and photochemically efficient probes for two-photon photolysis in living brain slices. The protocol only requires basic organic synthesis equipment, and no silica gel column chromatography or NMR spectroscopy is needed at any stage. HPLC is used to purify the caged transmitters at the end of the syntheses. Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestly equipped chemistry laboratory.

AB - This protocol describes a method for efficient chemical synthesis of dinitroindolinyl derivatives of glutamate and Î 3-aminobutyric acid. These caged neurotransmitters are currently the most chemically and photochemically efficient probes for two-photon photolysis in living brain slices. The protocol only requires basic organic synthesis equipment, and no silica gel column chromatography or NMR spectroscopy is needed at any stage. HPLC is used to purify the caged transmitters at the end of the syntheses. Thus, the synthesis of dinitroindolinyl-caged neurotransmitters is within the scope of a modestly equipped chemistry laboratory.

UR - http://www.scopus.com/inward/record.url?scp=79952377953&partnerID=8YFLogxK

U2 - 10.1038/nprot.2010.193

DO - 10.1038/nprot.2010.193

M3 - Article

C2 - 21372812

AN - SCOPUS:79952377953

SN - 1754-2189

VL - 6

SP - 314

EP - 326

JO - Nature Protocols

JF - Nature Protocols

IS - 3

ER -

A practical guide to the synthesis of dinitroindolinyl-caged neurotransmitters

Abstract

Access to Document

Fingerprint

Cite this