TY - JOUR
T1 - A positive role for Myc in TGFβ-induced Snail transcription and epithelial-to-mesenchymal transition
AU - Smith, A. P.
AU - Verrecchia, A.
AU - Fagà, G.
AU - Doni, M.
AU - Perna, D.
AU - Martinato, F.
AU - Guccione, E.
AU - Amati, B.
N1 - Funding Information:
We thank Gioacchino Natoli and Stefano Campaner for critical reading of the paper; all members of the Amati lab for discussion and feedback throughout this work; Giuseppina Giardina for help with lentiviruses; Gabriele Bucci for bioinformatic support; Peter ten Dijke, Thordur Oskarsson and Andreas Trumpp for cell lines; Pier Giuseppe Pelicci for his continuous support and enthusiasm. This work was supported by grants from the Italian Association for Cancer Research (AIRC) and the International Association for Cancer Research (AICR).
PY - 2009/1/22
Y1 - 2009/1/22
N2 - Myc and transforming growth factor-β (TGFβ) signaling are mutually antagonistic, that is Myc suppresses the activation of TGFβ-induced genes, whereas TGFβ represses c-myc transcription. Here, we report a positive role for Myc in the TGFβ response, consisting in the induction of an epithelial-to-mesenchymal transition (EMT) and the activation of the EMT-associated gene Snail. Knockdown of either Myc or the TGFβ effectors SMAD3/4 in epithelial cells eliminated Snail induction by TGFβ. Both Myc and SMAD complexes targeted the Snail promoter in vivo, DNA binding occurring in a mutually independent manner. Myc was bound prior to TGFβ treatment, and was required for rapid Snail activation upon SMAD binding induced by TGFβ. On the other hand, c-myc downregulation by TGFβ was a slower event, occurring after Snail induction. The response of Snail to another cytokine, hepatocyte growth factor (HGF), also depended on Myc and SMAD4. Thus, contrary to their antagonistic effects on Cip1 and INK4b, Myc and SMADs cooperate in signal-dependent activation of Snail in epithelial cells. Although Myc also targeted the Snail promoter in serum-stimulated fibroblasts, it was dispensable for its activation in these conditions, further illustrating that the action of Myc in transcriptional regulation is context-dependent. Our findings suggest that Myc and TGFβ signaling may cooperate in promoting EMT and metastasis in carcinomas.
AB - Myc and transforming growth factor-β (TGFβ) signaling are mutually antagonistic, that is Myc suppresses the activation of TGFβ-induced genes, whereas TGFβ represses c-myc transcription. Here, we report a positive role for Myc in the TGFβ response, consisting in the induction of an epithelial-to-mesenchymal transition (EMT) and the activation of the EMT-associated gene Snail. Knockdown of either Myc or the TGFβ effectors SMAD3/4 in epithelial cells eliminated Snail induction by TGFβ. Both Myc and SMAD complexes targeted the Snail promoter in vivo, DNA binding occurring in a mutually independent manner. Myc was bound prior to TGFβ treatment, and was required for rapid Snail activation upon SMAD binding induced by TGFβ. On the other hand, c-myc downregulation by TGFβ was a slower event, occurring after Snail induction. The response of Snail to another cytokine, hepatocyte growth factor (HGF), also depended on Myc and SMAD4. Thus, contrary to their antagonistic effects on Cip1 and INK4b, Myc and SMADs cooperate in signal-dependent activation of Snail in epithelial cells. Although Myc also targeted the Snail promoter in serum-stimulated fibroblasts, it was dispensable for its activation in these conditions, further illustrating that the action of Myc in transcriptional regulation is context-dependent. Our findings suggest that Myc and TGFβ signaling may cooperate in promoting EMT and metastasis in carcinomas.
KW - EMT
KW - Myc
KW - Snail
KW - TGFb
UR - http://www.scopus.com/inward/record.url?scp=59649128301&partnerID=8YFLogxK
U2 - 10.1038/onc.2008.395
DO - 10.1038/onc.2008.395
M3 - Article
C2 - 18978814
AN - SCOPUS:59649128301
SN - 0950-9232
VL - 28
SP - 422
EP - 430
JO - Oncogene
JF - Oncogene
IS - 3
ER -