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A physical and transcriptional map of the preaxial polydactyly locus on chromosome 7q36

  • Henk C. Heus
  • , Anne Hing
  • , Marijke J. Van Baren
  • , Marijke Joosse
  • , Guido J. Breedveld
  • , Jen C. Wang
  • , Andrea Burgess
  • , Helen Donnis-Keller
  • , Cathleen Berglund
  • , Julia Zguricas
  • , Stephen W. Scherer
  • , Johanna M. Rommens
  • , Ben A. Oostra
  • , Peter Heutink

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Preaxial polydactyly is a congenital hand malformation that includes duplicated thumbs, various forms of triphalangeal thumbs, and duplications of the index finger. A locus for preaxial polydactyly has been mapped to a region of 1.9 cM on chromosome 7q36 between polymorphic markers D7S550 and D7S2423. We constructed a detailed physical map of the preaxial polydactyly candidate region. With a combination of methods we identified and positioned 11 transcripts within this map. By recombination analysis on families with preaxial polydactyly, using newly developed polymorphic markers, we were able to reduce the candidate region to approximately 450 kb. The homeobox gene HLXB9, a putative receptor C7orf2, and two transcripts of unknown function, C7orf3 and C7orf4, map in the refined candidate region and have been subjected to mutation analysis in individuals with preaxial polydactyly.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalGenomics
Volume57
Issue number3
DOIs
StatePublished - 1 May 1999
Externally publishedYes

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