A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89

Prashant S. Phale, Milind C. Mahajan, Chelakard S. Vaidyanathan

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalArchives of Microbiology
Volume163
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

Keywords

  • 1-Naphthoic acid
  • Hydrocarbon degradation
  • Hydroxylation
  • Pseudomonas
  • Ring cleavage

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