Abstract
Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.
Original language | English |
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Pages (from-to) | 42-47 |
Number of pages | 6 |
Journal | Archives of Microbiology |
Volume | 163 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1995 |
Externally published | Yes |
Keywords
- 1-Naphthoic acid
- Hydrocarbon degradation
- Hydroxylation
- Pseudomonas
- Ring cleavage