A novel fluorescent pH indicator for the acidic range

S. Marchesini; S. Gatt; V. Agmon; M. L. Giudici; E. Monti

A novel fluorescent pH indicator for the acidic range

S. Marchesini
, S. Gatt
, V. Agmon
, M. L. Giudici
, E. Monti

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Reaction of a commercial preparation of lissamine-rhodamine B sulfonyl chloride with primary amines gives rise to two sulfonamide derivatives, of which one behaves as a pH indicator with maximal fluorescence emission in the acidic range and a pKa of about 4.6. The structure of this derivative and the mechanism of fluorescence dependence on pH, elucidated by means of NMR, is presented.

Original language	English
Pages (from-to)	545-550
Number of pages	6
Journal	Biochemistry International
Volume	27
Issue number	3
State	Published - 1992
Externally published	Yes

Cite this

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title = "A novel fluorescent pH indicator for the acidic range",

abstract = "Reaction of a commercial preparation of lissamine-rhodamine B sulfonyl chloride with primary amines gives rise to two sulfonamide derivatives, of which one behaves as a pH indicator with maximal fluorescence emission in the acidic range and a pKa of about 4.6. The structure of this derivative and the mechanism of fluorescence dependence on pH, elucidated by means of NMR, is presented.",

author = "S. Marchesini and S. Gatt and V. Agmon and Giudici, \{M. L.\} and E. Monti",

year = "1992",

language = "English",

volume = "27",

pages = "545--550",

journal = "Biochemistry International",

issn = "0158-5231",

publisher = "John Wiley and Sons Inc.",

number = "3",

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TY - JOUR

T1 - A novel fluorescent pH indicator for the acidic range

AU - Marchesini, S.

AU - Gatt, S.

AU - Agmon, V.

AU - Giudici, M. L.

AU - Monti, E.

PY - 1992

Y1 - 1992

N2 - Reaction of a commercial preparation of lissamine-rhodamine B sulfonyl chloride with primary amines gives rise to two sulfonamide derivatives, of which one behaves as a pH indicator with maximal fluorescence emission in the acidic range and a pKa of about 4.6. The structure of this derivative and the mechanism of fluorescence dependence on pH, elucidated by means of NMR, is presented.

AB - Reaction of a commercial preparation of lissamine-rhodamine B sulfonyl chloride with primary amines gives rise to two sulfonamide derivatives, of which one behaves as a pH indicator with maximal fluorescence emission in the acidic range and a pKa of about 4.6. The structure of this derivative and the mechanism of fluorescence dependence on pH, elucidated by means of NMR, is presented.

UR - https://www.scopus.com/pages/publications/0026705349

M3 - Article

C2 - 1417891

AN - SCOPUS:0026705349

SN - 0158-5231

VL - 27

SP - 545

EP - 550

JO - Biochemistry International

JF - Biochemistry International

IS - 3

ER -

A novel fluorescent pH indicator for the acidic range

Abstract

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