A non-alkalophilic mutant strain of Bacillus alcalophilus grows on L-malate over a pH range from 5.0 to 9.0. The mutant does not exhibit the energy-dependent efflux of Na+ that has been used to assay a Na+ H+ antiporter in the wild type organism. The mutant also fails to transport α-aminoisobutyric acid, at pH 9.0, either in the presence or absence of Na+; at pH 5.5, the amino acid analogue is taken up by a Na+-independent mechanism. The properties of the mutant constitute strong evidence that the Na+ H+ antiporter is involved in maintaining an acidified cytoplasm in B. alcalophilus.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 14 Nov 1979|