TY - JOUR
T1 - A new recycling technique for human placental cotyledon perfusion
T2 - Application to studies of the fetomaternal transfer of glucose, inulin, and antipyrine
AU - Brandes, J. M.
AU - Tavoloni, N.
AU - Potter, B. J.
AU - Sarkozi, L.
AU - Shepard, M. D.
AU - Berk, P. D.
N1 - Funding Information:
From the Departments of Medicine (Polly Annenberg Levee Hematol-ogy Center) and Chemistry, Mount Sinai School of Medicine, and the Department of Obstetrics and Gynecology A, Rambam Medical Center, Technion (Israel Institute of Technology). Supported in part by Grant AM 26438 from the National Institute of Arthritis, Diabetes, Digestive and Kidney Disease and generous gifts from the Jack Martin Fund and the Polly Annenberg Levee Charitable Trust. Received for publication August 11, 1982. Revised january 28, 1983. Accepted March 3, 1983. Reprint requests: Paul D. Berk, M.D., Polly Annenberg Levee Hematolor;y Center, Mount Sinai School of Medicine, 1 GusfLLve L. Levy Place, New York, New York 10029. *Polly Annenberg Levee Visiting Scholar of the Mount Sinai School of Medicine.
PY - 1983
Y1 - 1983
N2 - A previously described technique has been modified to permit the continuously recirculating perfusion of the separate maternal and fetal circulation of an isolated cotyledon of human placeta. Viability of the perfused cotyledons was established by measurements of oxygen consumption (average, 0.18 ml/gm/hr), glucose utilization (average, 1.0 mg/gm/hr), and lactate production (<0.01 μmol/gm/hr), and integrity of the placental barrier by the failure of India ink, 125I-albumin, or 35S-sulfobromophthalein to cross from fetal to maternal circulation. Clearance of 3H-inulin from the fetal circuit, 0.0059 ± 0.0005 (SE) ml/min/gm, coresponded to 2.5% of its clearance by the adult human kidney. Clearance of 14C-antipyrine was 0.013 ± 0.003 ml/min/gm. After introduction into the fetal circuit, the observed appearance of both inulin and antipyrine in the maternal circuit paralleled curves predicted by a simple mathematical model. The use of a continuously recirculation perfusion system is technically feasible, and has advantages over the single-pass technique for studying transplacental transfer of metabolites with low efficiency of extraction.
AB - A previously described technique has been modified to permit the continuously recirculating perfusion of the separate maternal and fetal circulation of an isolated cotyledon of human placeta. Viability of the perfused cotyledons was established by measurements of oxygen consumption (average, 0.18 ml/gm/hr), glucose utilization (average, 1.0 mg/gm/hr), and lactate production (<0.01 μmol/gm/hr), and integrity of the placental barrier by the failure of India ink, 125I-albumin, or 35S-sulfobromophthalein to cross from fetal to maternal circulation. Clearance of 3H-inulin from the fetal circuit, 0.0059 ± 0.0005 (SE) ml/min/gm, coresponded to 2.5% of its clearance by the adult human kidney. Clearance of 14C-antipyrine was 0.013 ± 0.003 ml/min/gm. After introduction into the fetal circuit, the observed appearance of both inulin and antipyrine in the maternal circuit paralleled curves predicted by a simple mathematical model. The use of a continuously recirculation perfusion system is technically feasible, and has advantages over the single-pass technique for studying transplacental transfer of metabolites with low efficiency of extraction.
UR - http://www.scopus.com/inward/record.url?scp=0020616252&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(83)91081-5
DO - 10.1016/0002-9378(83)91081-5
M3 - Article
C2 - 6869452
AN - SCOPUS:0020616252
VL - 146
SP - 800
EP - 806
JO - Unknown Journal
JF - Unknown Journal
IS - 7
ER -