TY - JOUR
T1 - A new continuous, monodimensional electrophoretic system for the separation and quantitation of individual glycosaminoglycans
AU - Schuchman, Edward H.
AU - Desnick, Robert J.
N1 - Funding Information:
We wish to thank Ms. Lorri Jagendorf for assisting in the development of this assay and Ms. Linda Lugo for expert clerical assistance. This investigation was supported in part by a grant (I-578) from the March of Dimes Birth Defects Foundation and by grants (GM-25279 and AM-25759) from the National Institutes of Health. E.H.S. is the recipient of a predoctoral fellowship (1 T32 HD0705) from the National Institutes of Health.
PY - 1981/11/1
Y1 - 1981/11/1
N2 - A new continuous, monodimensional cellulose acetate electrophoretic system was developed for the resolution of six major mammalian glycosaminoglycans. Keratan sulfate, heparan sulfate, dermatan sulfate, heparin, chondroitin 4-sulfate, and hyaluronic acid were reproductibly separated, presumably on the basis of their differential binding to the amino groups on ethylenediaminetetraacetic acid and lithium cations in the same electrophoretic solution. This micromethod permits the rapid separation, direct comparison, and quantitative determination of these individual glycosaminoglycans following a single electrophoretic procedure.
AB - A new continuous, monodimensional cellulose acetate electrophoretic system was developed for the resolution of six major mammalian glycosaminoglycans. Keratan sulfate, heparan sulfate, dermatan sulfate, heparin, chondroitin 4-sulfate, and hyaluronic acid were reproductibly separated, presumably on the basis of their differential binding to the amino groups on ethylenediaminetetraacetic acid and lithium cations in the same electrophoretic solution. This micromethod permits the rapid separation, direct comparison, and quantitative determination of these individual glycosaminoglycans following a single electrophoretic procedure.
UR - https://www.scopus.com/pages/publications/0019812355
U2 - 10.1016/0003-2697(81)90801-0
DO - 10.1016/0003-2697(81)90801-0
M3 - Article
C2 - 6275743
AN - SCOPUS:0019812355
SN - 0003-2697
VL - 117
SP - 419
EP - 426
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -