A new continuous, monodimensional electrophoretic system for the separation and quantitation of individual glycosaminoglycans

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Abstract

A new continuous, monodimensional cellulose acetate electrophoretic system was developed for the resolution of six major mammalian glycosaminoglycans. Keratan sulfate, heparan sulfate, dermatan sulfate, heparin, chondroitin 4-sulfate, and hyaluronic acid were reproductibly separated, presumably on the basis of their differential binding to the amino groups on ethylenediaminetetraacetic acid and lithium cations in the same electrophoretic solution. This micromethod permits the rapid separation, direct comparison, and quantitative determination of these individual glycosaminoglycans following a single electrophoretic procedure.

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalAnalytical Biochemistry
Volume117
Issue number2
DOIs
StatePublished - 1 Nov 1981

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