A multiplex RT-PCR assay for detection and differentiation of avian-origin canine H3N2, equine-origin H3N8, human-origin H3N2, and H1N1/2009 canine influenza viruses

Chenxi Wang, Qian Wang, Junyi Hu, Honglei Sun, Juan Pu, Jinhua Liu, Yipeng Sun

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Virological and serological surveys have documented that H1N1/2009, avian-origin canine H3N2 (cH3N2), seasonal human-origin H3N2 (hH3N2), and equine-origin H3N8 influenza viruses are consistently circulating in dogs. In the present study, a multiplex reverse-tran-scriptase polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection and differentiation of these influenza viruses. Four primer sets were designed to target the hemagglutinin genes of H1N1/2009, cH3N2, hH3N2, and H3N8 canine influenza viruses (CIVs). This mRT-PCR assay demonstrated high specificity and sensitivity for the four CIV subtypes. Additionally, mRT-PCR results obtained from 420 clinical samples were consistent with those obtained by the conventional virus isolation method. Our mRT-PCR assay is reliable for clinical diagnosis and rapid identification of CIVs.

Original languageEnglish
Article numbere0170374
JournalPLoS ONE
Volume12
Issue number1
DOIs
StatePublished - Jan 2017
Externally publishedYes

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