TY - JOUR
T1 - A multiplex RT-PCR assay for detection and differentiation of avian-origin canine H3N2, equine-origin H3N8, human-origin H3N2, and H1N1/2009 canine influenza viruses
AU - Wang, Chenxi
AU - Wang, Qian
AU - Hu, Junyi
AU - Sun, Honglei
AU - Pu, Juan
AU - Liu, Jinhua
AU - Sun, Yipeng
N1 - Publisher Copyright:
© 2017 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2017/1
Y1 - 2017/1
N2 - Virological and serological surveys have documented that H1N1/2009, avian-origin canine H3N2 (cH3N2), seasonal human-origin H3N2 (hH3N2), and equine-origin H3N8 influenza viruses are consistently circulating in dogs. In the present study, a multiplex reverse-tran-scriptase polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection and differentiation of these influenza viruses. Four primer sets were designed to target the hemagglutinin genes of H1N1/2009, cH3N2, hH3N2, and H3N8 canine influenza viruses (CIVs). This mRT-PCR assay demonstrated high specificity and sensitivity for the four CIV subtypes. Additionally, mRT-PCR results obtained from 420 clinical samples were consistent with those obtained by the conventional virus isolation method. Our mRT-PCR assay is reliable for clinical diagnosis and rapid identification of CIVs.
AB - Virological and serological surveys have documented that H1N1/2009, avian-origin canine H3N2 (cH3N2), seasonal human-origin H3N2 (hH3N2), and equine-origin H3N8 influenza viruses are consistently circulating in dogs. In the present study, a multiplex reverse-tran-scriptase polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection and differentiation of these influenza viruses. Four primer sets were designed to target the hemagglutinin genes of H1N1/2009, cH3N2, hH3N2, and H3N8 canine influenza viruses (CIVs). This mRT-PCR assay demonstrated high specificity and sensitivity for the four CIV subtypes. Additionally, mRT-PCR results obtained from 420 clinical samples were consistent with those obtained by the conventional virus isolation method. Our mRT-PCR assay is reliable for clinical diagnosis and rapid identification of CIVs.
UR - http://www.scopus.com/inward/record.url?scp=85010441806&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0170374
DO - 10.1371/journal.pone.0170374
M3 - Article
C2 - 28107507
AN - SCOPUS:85010441806
SN - 1932-6203
VL - 12
JO - PLoS ONE
JF - PLoS ONE
IS - 1
M1 - e0170374
ER -